NGS Details (SRR3458562_Metagenome_example): Enterobacteria phage SfI

Assembly

12945 (54 contig(s))
6.9
840
54.20 rpm (after QC)
0
read mapping against reference + variant calling
Bcf Tools

Coverage Map

Variant Tables

Assignment

Enterobacteria phage SfI (Taxonomy ID: 1225789)
99.6833
98.7995
25
65

Alignment

25726.0 (NT) + 28044.0 (AA) = 53770.0
99.5188
Local, heuristic, nucleotide (BLASTN)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT883836833.7%2572699.4%12945 (100%)12904 (99.7%)0/0
Mutations:3437A>G, 3443A>T, 3596G>C, 3609A>G, 5150C>G, 5261G>T, 5269C>G, 5507C>G, 6955C>T, 8940C>T, 9816G>A, 9853A>T, 11389T>C, 11395A>G, 11488C>G, 11494G>T, 11644G>A, 11664C>A, 11671A>G, 11777A>G, 11785G>A, 11788C>T, 12544A>G, 12717A>C, 13026C>T, 13028T>C, 14034C>G, 14040G>T, 14053A>C, 14154T>C, 14193T>C, 14645C>T, 14775G>A, 15195G>T, 15268T>A, 16417A>C, 16443C>A, 16510T>C, 16522C>T, 35280T>G, 35311G>A
CDS
1416532.7%32893.2%54 (100%)53 (98.1%)0/0/0/01
157851.9%210498.4%300 (100%)298 (99.3%)0/0/0/01
16188.5%34597.7%54 (100%)53 (98.1%)0/0/0/01
135844.6%111496.3%166 (100%)162 (97.6%)0/0/0/00
Protein mutations:N339D (3609A>G)
Codon mutations:GGA281GGG (3437A>G), GTA283GTT (3443A>T), CCG334CCC (3596G>C), AAT339GAT (3609A>G)
122990.4%136099.1%207 (100%)206 (99.5%)0/0/0/01
140235.1%89498.5%141 (100%)140 (99.3%)0/0/0/01
Protein mutations:T306S (5269C>G)
Codon mutations:ACC266ACG (5150C>G), CTG303CTT (5261G>T), ACC306AGC (5269C>G), GGC385GGG (5507C>G)
167100%405100%67 (100%)67 (100%)0/0/0/01
1313763.5%63897.9%87 (100%)87 (100%)0/0/0/01
116993.5%113297.6%158 (100%)157 (99.4%)0/0/1/01
17925.1%30393.8%47 (100%)45 (95.7%)0/0/0/00
13249955.5%179598.4%277 (100%)276 (99.6%)0/0/0/01
1119100%820100%119 (100%)119 (100%)0/0/0/01
190100%57499.3%90 (100%)89 (98.9%)0/0/0/01
Protein mutations:S65N (9816G>A)
Codon mutations:AGC65AAC (9816G>A), GCA77GCT (9853A>T)
1661253.1%214096.4%325 (100%)318 (97.8%)0/0/0/01
Protein mutations:N485K (11488C>G), P544Q (11664C>A), M582V (11777A>G)
Codon mutations:TTT452TTC (11389T>C), AAA454AAG (11395A>G), AAC485AAG (11488C>G), GGG487GGT (11494G>T), GCG537GCA (11644G>A), CCG544CAG (11664C>A), GTA546GTG (11671A>G), ATG582GTG (11777A>G), TCG584TCA (11785G>A), CCC585CCT (11788C>T)
140991.3%269399.6%409 (100%)407 (99.5%)0/0/0/00
Protein mutations:N268T (12717A>C), A371V (13026C>T)
Codon mutations:CCA210CCG (12544A>G), AAC268ACC (12717A>C), GCG371GTG (13026C>T), TTG372CTG (13028T>C)
4035670.6%176896.5%254 (100%)251 (98.8%)0/0/0/00
5718365.0%77295.9%119 (100%)117 (98.3%)0/0/0/01
Protein mutations:G148S (14775G>A)
Codon mutations:TCC104TCT (14645C>T), GGC148AGC (14775G>A)
1142100%105898.3%142 (100%)140 (98.6%)0/0/0/01
Protein mutations:R105L (15195G>T), F129L (15268T>A)
Codon mutations:CGC105CTC (15195G>T), TTT129TTA (15268T>A)
135343.6%103497.8%154 (100%)153 (99.4%)0/0/0/01
119559.5%79995.8%116 (100%)113 (97.4%)0/0/0/01
Protein mutations:R25S (16417A>C), P34Q (16443C>A)
Codon mutations:AGA25AGC (16417A>C), CCG34CAG (16443C>A), ACT56ACC (16510T>C), AAC60AAT (16522C>T)
12310.6%15094.3%23 (100%)22 (95.7%)0/0/0/00
34338811.9%310100%46 (100%)46 (100%)0/0/0/01
72534.5%12285.9%19 (100%)18 (94.7%)0/0/0/00
16830.0%47698.1%68 (100%)67 (98.5%)0/0/0/00
14326223.3%39496.1%61 (100%)60 (98.4%)0/0/0/01
111643.2%39993.4%60 (100%)57 (95.0%)0/0/0/00
19070.0%38598.0%63 (100%)62 (98.4%)0/0/0/01
14480.0%32198.8%44 (100%)43 (97.7%)0/0/0/00
5112335.8%25093.3%44 (100%)43 (97.7%)0/0/0/01
14114.6%26398.5%41 (100%)40 (97.6%)0/0/0/00
19023820.6%36498.4%49 (100%)49 (100%)0/0/0/01
246340.8%26996.1%40 (100%)39 (97.5%)0/0/0/00
1827640.0%85697.4%120 (100%)119 (99.2%)0/0/0/00
91977.2%40100%7 (100%)7 (100%)0/0/0/01
127100%170100%27 (100%)27 (100%)0/0/0/01
1149.5%94100%14 (100%)14 (100%)0/0/0/00
171915.8%2586.2%3 (100%)3 (100%)0/0/0/01
18049.1%56699.1%80 (100%)78 (97.5%)0/0/0/00
Protein mutations:V52I (35311G>A)
Codon mutations:GCT41GCG (35280T>G), GTT52ATT (35311G>A)
4811759.8%514100%70 (100%)70 (100%)0/0/0/01
Proteins
1416532.7%32893.2%54 (100%)53 (98.1%)0/0/0/01
Protein mutations:none
Codon mutations:none
157851.9%210498.4%300 (100%)298 (99.3%)0/0/0/01
Protein mutations:none
Codon mutations:none
16188.5%34597.7%54 (100%)53 (98.1%)0/0/0/01
Protein mutations:none
Codon mutations:none
135844.6%111496.3%166 (100%)162 (97.6%)0/0/0/00
Protein mutations:N339D (3609A>G)
Codon mutations:GGA281GGG (3437A>G), GTA283GTT (3443A>T), CCG334CCC (3596G>C), AAT339GAT (3609A>G)
122990.4%136099.1%207 (100%)206 (99.5%)0/0/0/01
Protein mutations:none
Codon mutations:none
140235.1%89498.5%141 (100%)140 (99.3%)0/0/0/01
Protein mutations:T306S (5269C>G)
Codon mutations:ACC266ACG (5150C>G), CTG303CTT (5261G>T), ACC306AGC (5269C>G), GGC385GGG (5507C>G)
167100%405100%67 (100%)67 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
1313763.5%63897.9%87 (100%)87 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
116993.5%113297.6%158 (100%)157 (99.4%)0/0/1/01
Protein mutations:none
Codon mutations:TTC137TTT (6955C>T)
17925.1%30393.8%47 (100%)45 (95.7%)0/0/0/00
Protein mutations:none
Codon mutations:none
13249955.5%179598.4%277 (100%)276 (99.6%)0/0/0/01
Protein mutations:none
Codon mutations:AGC390AGT (8940C>T)
1119100%820100%119 (100%)119 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
190100%57499.3%90 (100%)89 (98.9%)0/0/0/01
Protein mutations:S65N (9816G>A)
Codon mutations:AGC65AAC (9816G>A), GCA77GCT (9853A>T)
1661253.1%214096.4%325 (100%)318 (97.8%)0/0/0/01
Protein mutations:N485K (11488C>G), P544Q (11664C>A), M582V (11777A>G)
Codon mutations:TTT452TTC (11389T>C), AAA454AAG (11395A>G), AAC485AAG (11488C>G), GGG487GGT (11494G>T), GCG537GCA (11644G>A), CCG544CAG (11664C>A), GTA546GTG (11671A>G), ATG582GTG (11777A>G), TCG584TCA (11785G>A), CCC585CCT (11788C>T)
140991.3%269399.6%409 (100%)407 (99.5%)0/0/0/00
Protein mutations:N268T (12717A>C), A371V (13026C>T)
Codon mutations:CCA210CCG (12544A>G), AAC268ACC (12717A>C), GCG371GTG (13026C>T), TTG372CTG (13028T>C)
4035670.6%176896.5%254 (100%)251 (98.8%)0/0/0/00
Protein mutations:none
Codon mutations:GCC260GCG (14034C>G), GCG262GCT (14040G>T), AGA267CGA (14053A>C), TTT300TTC (14154T>C), GTT313GTC (14193T>C)
5718365.0%77295.9%119 (100%)117 (98.3%)0/0/0/01
Protein mutations:G148S (14775G>A)
Codon mutations:TCC104TCT (14645C>T), GGC148AGC (14775G>A)
1142100%105898.3%142 (100%)140 (98.6%)0/0/0/01
Protein mutations:R105L (15195G>T), F129L (15268T>A)
Codon mutations:CGC105CTC (15195G>T), TTT129TTA (15268T>A)
135343.6%103497.8%154 (100%)153 (99.4%)0/0/0/01
Protein mutations:none
Codon mutations:none
119559.5%79995.8%116 (100%)113 (97.4%)0/0/0/01
Protein mutations:R25S (16417A>C), P34Q (16443C>A)
Codon mutations:AGA25AGC (16417A>C), CCG34CAG (16443C>A), ACT56ACC (16510T>C), AAC60AAT (16522C>T)
12310.6%15094.3%23 (100%)22 (95.7%)0/0/0/00
Protein mutations:none
Codon mutations:none
34338811.9%310100%46 (100%)46 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
72534.5%12285.9%19 (100%)18 (94.7%)0/0/0/00
Protein mutations:none
Codon mutations:none
16830.0%47698.1%68 (100%)67 (98.5%)0/0/0/00
Protein mutations:none
Codon mutations:none
14326223.3%39496.1%61 (100%)60 (98.4%)0/0/0/01
Protein mutations:none
Codon mutations:none
111643.2%39993.4%60 (100%)57 (95.0%)0/0/0/00
Protein mutations:none
Codon mutations:none
19070.0%38598.0%63 (100%)62 (98.4%)0/0/0/01
Protein mutations:none
Codon mutations:none
14480.0%32198.8%44 (100%)43 (97.7%)0/0/0/00
Protein mutations:none
Codon mutations:none
5112335.8%25093.3%44 (100%)43 (97.7%)0/0/0/01
Protein mutations:none
Codon mutations:none
14114.6%26398.5%41 (100%)40 (97.6%)0/0/0/00
Protein mutations:none
Codon mutations:none
19023820.6%36498.4%49 (100%)49 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
246340.8%26996.1%40 (100%)39 (97.5%)0/0/0/00
Protein mutations:none
Codon mutations:none
1827640.0%85697.4%120 (100%)119 (99.2%)0/0/0/00
Protein mutations:none
Codon mutations:none
91977.2%40100%7 (100%)7 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
127100%170100%27 (100%)27 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
1149.5%94100%14 (100%)14 (100%)0/0/0/00
Protein mutations:none
Codon mutations:none
171915.8%2586.2%3 (100%)3 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
18049.1%56699.1%80 (100%)78 (97.5%)0/0/0/00
Protein mutations:V52I (35311G>A)
Codon mutations:GCT41GCG (35280T>G), GTT52ATT (35311G>A)
4811759.8%514100%70 (100%)70 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43