NGS Details (SRR3458562_Metagenome_example): Peduovirus P2

Assembly

9435 (37 contig(s))
6.8
575
37.10 rpm (after QC)
0
read mapping against reference + variant calling
Bcf Tools

Coverage Map

Variant Tables

Assignment

Peduovirus P2 (Taxonomy ID: 10679)
99.7244
98.8068
13
43

Alignment

18766.0 (NT) + 20119.0 (AA) = 38885.0
99.6361
Local, heuristic, nucleotide (BLASTN)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT16463028128.1%1876699.4%9435 (100%)9409 (99.7%)0/0
Mutations:3343G>A, 3367G>T, 3697C>A, 3708C>T, 4826C>T, 17901A>G, 17916G>T, 17981T>C, 18012A>C, 18059C>T, 18350G>A, 18467C>A, 20072G>C, 20113T>C, 20137C>G, 24798G>A, 24981T>G, 25017T>C, 25164C>G, 29020T>G, 29167A>G, 29710C>T, 29719T>C, 29731G>C, 30059A>C, 30154G>A
CDS
14506.8%29590.5%40 (100%)38 (95.0%)0/0/0/00
127195.1%176999.6%271 (100%)269 (99.3%)0/0/0/00
Protein mutations:A181V (3708C>T)
Codon mutations:AAG59AAA (3343G>A), CTG67CTT (3367G>T), ACC177ACA (3697C>A), GCC181GTC (3708C>T)
5626950.3%119796.8%180 (100%)177 (98.3%)0/0/0/00
14318718.1%27096.4%45 (100%)44 (97.8%)0/0/0/00
467531.9%18695.4%30 (100%)29 (96.7%)0/0/0/00
14316212.0%13488.7%20 (100%)19 (95.0%)0/0/0/00
1421420.7%1100%1 (100%)1 (100%)0/0/0/01
514215.5%11296.6%22 (100%)22 (100%)0/0/0/01
469551.5%35998.4%50 (100%)50 (100%)0/0/0/00
1117.1%6293.9%11 (100%)10 (90.9%)0/0/0/00
17419912.3%17388.3%26 (100%)25 (96.2%)0/0/0/00
55443.1%32194.1%50 (100%)49 (98.0%)0/0/0/00
2012258.3%15593.4%25 (100%)25 (100%)0/0/0/00
366718.1%28597.3%32 (100%)31 (96.9%)0/0/0/00
8010213.1%16394.2%23 (100%)22 (95.7%)0/0/0/00
7535859.4%157797.6%236 (100%)232 (98.3%)0/0/0/00
Protein mutations:M84V (17901A>G), G89C (17916G>T), M121L (18012A>C)
Codon mutations:ATG84GTG (17901A>G), GGC89TGC (17916G>T), GAT110GAC (17981T>C), ATG121CTG (18012A>C), GGC136GGT (18059C>T), GTG233GTA (18350G>A), CGC272CGA (18467C>A)
4314370.6%64199.4%101 (100%)101 (100%)0/0/0/01
439254.3%29298.6%50 (100%)50 (100%)0/0/0/01
181639.8%201896.9%325 (100%)318 (97.8%)0/0/0/01
Protein mutations:E75Q (20072G>C)
Codon mutations:GAG75CAG (20072G>C), CGT88CGC (20113T>C), GTC96GTG (20137C>G)
116011.3%10389.6%18 (100%)17 (94.4%)0/0/0/01
138851.0%128898.8%198 (100%)196 (99.0%)0/0/0/01
11621.9%14097.2%16 (100%)15 (93.8%)0/0/0/00
1338100%2309100%338 (100%)338 (100%)0/0/0/01
1100100%668100%100 (100%)100 (100%)0/0/0/01
192100%640100%92 (100%)92 (100%)0/0/0/01
157100%381100%57 (100%)57 (100%)0/0/0/01
1137.8%77100%13 (100%)13 (100%)0/0/0/00
337557.3%31498.7%43 (100%)43 (100%)0/0/0/01
19269.6%42999.1%64 (100%)64 (100%)0/0/0/01
175070.9%376098.1%540 (100%)534 (98.9%)0/0/0/00
Protein mutations:E566D (29731G>C), M676L (30059A>C)
Codon mutations:CCT329CCG (29020T>G), AAA378AAG (29167A>G), GTC559GTT (29710C>T), AGT562AGC (29719T>C), GAG566GAC (29731G>C), ATG676CTG (30059A>C), CAG707CAA (30154G>A)
Proteins
14506.8%29590.5%40 (100%)38 (95.0%)0/0/0/00
Protein mutations:none
Codon mutations:none
127195.1%176999.6%271 (100%)269 (99.3%)0/0/0/00
Protein mutations:A181V (3708C>T)
Codon mutations:AAG59AAA (3343G>A), CTG67CTT (3367G>T), ACC177ACA (3697C>A), GCC181GTC (3708C>T)
5626950.3%119796.8%180 (100%)177 (98.3%)0/0/0/00
Protein mutations:none
Codon mutations:GTC249GTT (4826C>T)
14318718.1%27096.4%45 (100%)44 (97.8%)0/0/0/00
Protein mutations:none
Codon mutations:none
467531.9%18695.4%30 (100%)29 (96.7%)0/0/0/00
Protein mutations:none
Codon mutations:none
14316212.0%13488.7%20 (100%)19 (95.0%)0/0/0/00
Protein mutations:none
Codon mutations:none
1421420.7%1100%1 (100%)1 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
514215.5%11296.6%22 (100%)22 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
469551.5%35998.4%50 (100%)50 (100%)0/0/0/00
Protein mutations:none
Codon mutations:none
1117.1%6293.9%11 (100%)10 (90.9%)0/0/0/00
Protein mutations:none
Codon mutations:none
17419912.3%17388.3%26 (100%)25 (96.2%)0/0/0/00
Protein mutations:none
Codon mutations:none
55443.1%32194.1%50 (100%)49 (98.0%)0/0/0/00
Protein mutations:none
Codon mutations:none
2012258.3%15593.4%25 (100%)25 (100%)0/0/0/00
Protein mutations:none
Codon mutations:none
366718.1%28597.3%32 (100%)31 (96.9%)0/0/0/00
Protein mutations:none
Codon mutations:none
8010213.1%16394.2%23 (100%)22 (95.7%)0/0/0/00
Protein mutations:none
Codon mutations:none
7535859.4%157797.6%236 (100%)232 (98.3%)0/0/0/00
Protein mutations:M84V (17901A>G), G89C (17916G>T), M121L (18012A>C)
Codon mutations:ATG84GTG (17901A>G), GGC89TGC (17916G>T), GAT110GAC (17981T>C), ATG121CTG (18012A>C), GGC136GGT (18059C>T), GTG233GTA (18350G>A), CGC272CGA (18467C>A)
4314370.6%64199.4%101 (100%)101 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
439254.3%29298.6%50 (100%)50 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
181639.8%201896.9%325 (100%)318 (97.8%)0/0/0/01
Protein mutations:E75Q (20072G>C)
Codon mutations:GAG75CAG (20072G>C), CGT88CGC (20113T>C), GTC96GTG (20137C>G)
116011.3%10389.6%18 (100%)17 (94.4%)0/0/0/01
Protein mutations:none
Codon mutations:none
138851.0%128898.8%198 (100%)196 (99.0%)0/0/0/01
Protein mutations:none
Codon mutations:none
11621.9%14097.2%16 (100%)15 (93.8%)0/0/0/00
Protein mutations:none
Codon mutations:none
1338100%2309100%338 (100%)338 (100%)0/0/0/01
Protein mutations:none
Codon mutations:TCG104TCC (25164C>G), AAA153AAG (25017T>C), CTA165CTC (24981T>G), ATC226ATT (24798G>A)
1100100%668100%100 (100%)100 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
192100%640100%92 (100%)92 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
157100%381100%57 (100%)57 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
1137.8%77100%13 (100%)13 (100%)0/0/0/00
Protein mutations:none
Codon mutations:none
337557.3%31498.7%43 (100%)43 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
19269.6%42999.1%64 (100%)64 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
175070.9%376098.1%540 (100%)534 (98.9%)0/0/0/00
Protein mutations:E566D (29731G>C), M676L (30059A>C)
Codon mutations:CCT329CCG (29020T>G), AAA378AAG (29167A>G), GTC559GTT (29710C>T), AGT562AGC (29719T>C), GAG566GAC (29731G>C), ATG676CTG (30059A>C), CAG707CAA (30154G>A)
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43