NGS Details (SRR3458562_Metagenome_example): RD114 retrovirus

Assembly

893 (6 contig(s))
2.1
16
1.03 rpm (after QC)
10
de novo + reference guided assembly
Bcf Tools

Coverage Map

Variant Tables

Assignment

RD114 retrovirus (Taxonomy ID: 11834)
93.0571
90.625
3
2

Alignment

1558.0 (NT) + 1416.0 (AA) = 2974.0
84.4407
Global, seeded, nucleotide + amino acids (AGA)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT1769798810.6%155887.2%893 (100%)831 (93.1%)0/0
Mutations:1780G>A, 1786A>G, 1789C>T, 1803A>M, 1810C>T, 1831A>G, 1833T>Y, 1835C>T, 1847T>Y, 1849G>S, 1855A>C, 1860A>M, 1867A>M, 1900A>G, 2049G>R, 2050A>G, 2063A>M, 2068G>S, 2080A>G, 2083T>C, 2088T>W, 2089T>C, 2104A>G, 2123G>A, 2163C>A, 2164G>C, 2170A>G, 3496C>A, 3497C>T, 3500T>C, 3502A>C, 3511C>A, 3514C>G, 3515T>C, 3520C>T, 3526G>A, 3535C>T, 3538C>A, 3544T>C, 3545C>A, 3553T>C, 3559T>A, 3562C>A, 3566A>G, 3567G>A, 3574A>G, 3575A>G, 3576C>G, 3580C>G, 3582T>A, 3584C>A, 3586G>A, 3588C>G, 3589T>G, 3590C>G, 3592A>G, 6311A>C, 6314C>T, 6338C>T, 6370C>T, 7784A>C, 7983T>C
CDS
27517408.4%68566.8%147 (100%)126 (85.7%)0/0/9/02
Protein mutations:E393K (2123G>A), T406N (2163C>A 2164G>C), Q851* (3497C>T), L867I (3545C>A), R874E (3566A>G 3567G>A), T877G (3575A>G 3576C>G), D878E (3580C>G), F879Y (3582T>A), T881R (3588C>G 3589T>G), Q882E (3590C>G 3592A>G)
Codon mutations:CTG278CTA (1780G>A), GAA280GAG (1786A>G), TCC281TCT (1789C>T), CAC286CMC (1803A>M), CCC288CCT (1810C>T), CAA295CAG (1831A>G), CTC296CYC (1833T>Y), CTA297TTA (1835C>T), TTG301YTS (1847T>Y 1849G>S), ACA303ACC (1855A>C), GAA305GMA (1860A>M), CAA307CAM (1867A>M), CCA318CCG (1900A>G), GGA368GRG (2049G>R 2050A>G), AAT373MAT (2063A>M), TTG374TTS (2068G>S), AGA378AGG (2080A>G), ACT379ACC (2083T>C), ATT381AWC (2088T>W 2089T>C), GAA386GAG (2104A>G), GAA393AAA (2123G>A), ACG406AAC (2163C>A 2164G>C), GAA408GAG (2170A>G), GGC850GGA (3496C>A), CAA851TAA (3497C>T), TTA852CTC (3500T>C 3502A>C), ACC855ACA (3511C>A), CGC856CGG (3514C>G), TTA857CTA (3515T>C), CCC858CCT (3520C>T), GGG860GGA (3526G>A), AAC863AAT (3535C>T), TCC864TCA (3538C>A), ACT866ACC (3544T>C), CTC867ATC (3545C>A), GAT869GAC (3553T>C), GCT871GCA (3559T>A), CTC872CTA (3562C>A), AGG874GAG (3566A>G 3567G>A), CTA876CTG (3574A>G), ACT877GGT (3575A>G 3576C>G), GAC878GAG (3580C>G), TTC879TAC (3582T>A), CGG880AGA (3584C>A 3586G>A), ACT881AGG (3588C>G 3589T>G), CAA882GAG (3590C>G 3592A>G)
156619.3%73196.7%109 (100%)106 (97.2%)0/0/0/01
Protein mutations:T57I (6370C>T)
Codon mutations:CCA37CCC (6311A>C), TGC38TGT (6314C>T), TCC46TCT (6338C>T), ACT57ATT (6370C>T), CCA528CCC (7784A>C)
Proteins
27517408.4%68566.8%147 (100%)126 (85.7%)0/0/9/02
Protein mutations:E393K (2123G>A), T406N (2163C>A 2164G>C), Q851* (3497C>T), L867I (3545C>A), R874E (3566A>G 3567G>A), T877G (3575A>G 3576C>G), D878E (3580C>G), F879Y (3582T>A), T881R (3588C>G 3589T>G), Q882E (3590C>G 3592A>G)
Codon mutations:CTG278CTA (1780G>A), GAA280GAG (1786A>G), TCC281TCT (1789C>T), CAC286CMC (1803A>M), CCC288CCT (1810C>T), CAA295CAG (1831A>G), CTC296CYC (1833T>Y), CTA297TTA (1835C>T), TTG301YTS (1847T>Y 1849G>S), ACA303ACC (1855A>C), GAA305GMA (1860A>M), CAA307CAM (1867A>M), CCA318CCG (1900A>G), GGA368GRG (2049G>R 2050A>G), AAT373MAT (2063A>M), TTG374TTS (2068G>S), AGA378AGG (2080A>G), ACT379ACC (2083T>C), ATT381AWC (2088T>W 2089T>C), GAA386GAG (2104A>G), GAA393AAA (2123G>A), ACG406AAC (2163C>A 2164G>C), GAA408GAG (2170A>G), GGC850GGA (3496C>A), CAA851TAA (3497C>T), TTA852CTC (3500T>C 3502A>C), ACC855ACA (3511C>A), CGC856CGG (3514C>G), TTA857CTA (3515T>C), CCC858CCT (3520C>T), GGG860GGA (3526G>A), AAC863AAT (3535C>T), TCC864TCA (3538C>A), ACT866ACC (3544T>C), CTC867ATC (3545C>A), GAT869GAC (3553T>C), GCT871GCA (3559T>A), CTC872CTA (3562C>A), AGG874GAG (3566A>G 3567G>A), CTA876CTG (3574A>G), ACT877GGT (3575A>G 3576C>G), GAC878GAG (3580C>G), TTC879TAC (3582T>A), CGG880AGA (3584C>A 3586G>A), ACT881AGG (3588C>G 3589T>G), CAA882GAG (3590C>G 3592A>G)
156619.3%73196.7%109 (100%)106 (97.2%)0/0/0/01
Protein mutations:T57I (6370C>T)
Codon mutations:CCA37CCC (6311A>C), TGC38TGT (6314C>T), TCC46TCT (6338C>T), ACT57ATT (6370C>T), CCA528CCC (7784A>C)
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43