Version 2.22.2
Genome Detective Platform

NGS Details (SRR3458562_Metagenome_example): Peduovirus P22H1

Assembly

1295 (4 contig(s))
15.3
196
12.65 rpm (after QC)
1
de novo + reference guided assembly
Bcf Tools

Coverage Map

Variant Tables

Assignment

Peduovirus P22H1 (Taxonomy ID: 2844217)
91.5644
91.2935
0
45

Alignment

2170.0 (NT) + 2601.0 (AA) = 4771.0
88.401
Local, heuristic, nucleotide (BLASTN)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT564272694.0%217083.8%1295 (99.3%)1194 (91.6%)9/0
Mutations:
595A>G 637T>C 643A>G 772G>A 784G>A 786C>G 13317G>C 13318T>A 13320G>C 13326G>C 13327T>A 13328T>A 13329T>G 13338T>C 13339A>T 13344A>T 13346G>T 13350G>A 13356T>C 13359A>G 13362T>C 13363G>A 13365C>A 13371A>C 13372G>A 13374T>C 13375A>G 13378A>G 13380C>T 13381C>A 13382T>C 13383T>A 13388G>A 13392C>T 13393A>T 13394C>T 13395C>A 13397T>A 13404C>A 13407C>G 13411A>C 13412C>T 13413C>T 13416C>T 13417A>G 13422_13423insTTTAACGCG 13426A>G 13428C>G 13431A>G 13437G>A 13438G>C 13440A>G 13441G>A 13443C>T 13446C>T 13448C>A 13452G>A 13453T>A 13455T>C 13458A>T 13459C>T 13461T>G 13464C>G 13465C>T 13467G>A 13468A>G 13470T>G 13471C>T 13482C>T 13485T>C 13488T>A 13497G>A 13521C>A 13555A>C 13566T>C 13627G>T 13632T>C 13650C>T 13654A>T 13659G>A 13698G>C 13707A>C 13755A>G 13756G>A 13764A>T 13767A>G 13773C>T 13776T>C 16143G>A 16147C>T 16149T>C 16151T>C 16163C>T 16169A>G 16177A>G 16179T>A 16202T>G 16219T>A 16302C>T 16329C>T 27119C>M 27132C>T
CDS
13222828.1%69198.4%97 (100%)95 (97.9%)0/0/0/00
Protein mutations:
A154P (784G>A 786C>G)
Codon mutations:
GCC154CCT (784G>A 786C>G) GGC158GGT (772G>A) AAT201AAC (643A>G) GCA203GCG (637T>C) GAT217GAC (595A>G)
46262417.7%99585.9%163 (98.2%)139 (83.7%)3/0/0/00
Protein mutations:
L467I (13318T>A 13320G>C) F470K (13327T>A 13328T>A 13329T>G) N474Y (13339A>T) W476L (13346G>T) A482T (13363G>A 13365C>A) G485S (13372G>A 13374T>C) T486A (13375A>G) T487A (13378A>G 13380C>T) L488T (13381C>A 13382T>C 13383T>A) G490D (13388G>A) T492L (13393A>T 13394C>T 13395C>A) F493Y (13397T>A) T498L (13411A>C 13412C>T 13413C>T) S500G (13417A>G) S501_W502insFNA (13422_13423insTTTAACGCG) S503G (13426A>G 13428C>G) E507Q (13438G>C 13440A>G) V508I (13441G>A 13443C>T) T510N (13448C>A) Y512N (13453T>A 13455T>C) N517E (13468A>G 13470T>G) A570S (13627G>T) T579S (13654A>T) A613T (13756G>A)
Codon mutations:
GTG466GTC (13317G>C) TTG467ATC (13318T>A 13320G>C) CGG469CGC (13326G>C) TTT470AAG (13327T>A 13328T>A 13329T>G) GTT473GTC (13338T>C) AAT474TAT (13339A>T) GTA475GTT (13344A>T) TGG476TTG (13346G>T) CAG477CAA (13350G>A) TAT479TAC (13356T>C) ACA480ACG (13359A>G) ACT481ACC (13362T>C) GCC482ACA (13363G>A 13365C>A) ACA484ACC (13371A>C) GGT485AGC (13372G>A 13374T>C) ACA486GCA (13375A>G) ACC487GCT (13378A>G 13380C>T) CTT488ACA (13381C>A 13382T>C 13383T>A) GGC490GAC (13388G>A) CGC491CGT (13392C>T) ACC492TTA (13393A>T 13394C>T 13395C>A) TTT493TAT (13397T>A) CGC495CGA (13404C>A) GTC496GTG (13407C>G) ACC498CTT (13411A>C 13412C>T 13413C>T) GGC499GGT (13416C>T) AGC500GGC (13417A>G) TCA501_TGG502insTTTAACGCG (13422_13423insTTTAACGCG) AGC503GGG (13426A>G 13428C>G) GAA504GAG (13431A>G) CGG506CGA (13437G>A) GAA507CAG (13438G>C 13440A>G) GTC508ATT (13441G>A 13443C>T) TAC509TAT (13446C>T) ACC510AAC (13448C>A) TCG511TCA (13452G>A) TAT512AAC (13453T>A 13455T>C) TCA513TCT (13458A>T) CTT514TTG (13459C>T 13461T>G) CCC515CCG (13464C>G) CTG516TTA (13465C>T 13467G>A) AAT517GAG (13468A>G 13470T>G) CTG518TTG (13471C>T) GGC521GGT (13482C>T) GGT522GGC (13485T>C) GCT523GCA (13488T>A) AAG526AAA (13497G>A) ACC534ACA (13521C>A) AGG546CGG (13555A>C) AAT549AAC (13566T>C) GCA570TCA (13627G>T) GTT571GTC (13632T>C) TCC577TCT (13650C>T) ACG579TCG (13654A>T) GGG580GGA (13659G>A) GCG593GCC (13698G>C) CGA596CGC (13707A>C) ACA612ACG (13755A>G) GCA613ACA (13756G>A) CGA615CGT (13764A>T) GTA616GTG (13767A>G) ATC618ATT (13773C>T) ATT619ATC (13776T>C)
139100%27294.8%39 (100%)36 (92.3%)0/0/0/00
Protein mutations:
A3T (16329C>T) E12K (16302C>T) *39C (16219T>A)
Codon mutations:
GCA3ACA (16329C>T) GAG12AAG (16302C>T) TGA39TGT (16219T>A)
1399.8%25698.5%39 (100%)38 (97.4%)0/0/0/00
1181788.0%38788.4%61 (100%)59 (96.7%)0/0/1/00
Proteins
13222828.1%69198.4%97 (100%)95 (97.9%)0/0/0/00
Protein mutations:
A154P (784G>A 786C>G)
Codon mutations:
GCC154CCT (784G>A 786C>G) GGC158GGT (772G>A) AAT201AAC (643A>G) GCA203GCG (637T>C) GAT217GAC (595A>G)
46262417.7%99585.9%163 (98.2%)139 (83.7%)3/0/0/00
Protein mutations:
L467I (13318T>A 13320G>C) F470K (13327T>A 13328T>A 13329T>G) N474Y (13339A>T) W476L (13346G>T) A482T (13363G>A 13365C>A) G485S (13372G>A 13374T>C) T486A (13375A>G) T487A (13378A>G 13380C>T) L488T (13381C>A 13382T>C 13383T>A) G490D (13388G>A) T492L (13393A>T 13394C>T 13395C>A) F493Y (13397T>A) T498L (13411A>C 13412C>T 13413C>T) S500G (13417A>G) S501_W502insFNA (13422_13423insTTTAACGCG) S503G (13426A>G 13428C>G) E507Q (13438G>C 13440A>G) V508I (13441G>A 13443C>T) T510N (13448C>A) Y512N (13453T>A 13455T>C) N517E (13468A>G 13470T>G) A570S (13627G>T) T579S (13654A>T) A613T (13756G>A)
Codon mutations:
GTG466GTC (13317G>C) TTG467ATC (13318T>A 13320G>C) CGG469CGC (13326G>C) TTT470AAG (13327T>A 13328T>A 13329T>G) GTT473GTC (13338T>C) AAT474TAT (13339A>T) GTA475GTT (13344A>T) TGG476TTG (13346G>T) CAG477CAA (13350G>A) TAT479TAC (13356T>C) ACA480ACG (13359A>G) ACT481ACC (13362T>C) GCC482ACA (13363G>A 13365C>A) ACA484ACC (13371A>C) GGT485AGC (13372G>A 13374T>C) ACA486GCA (13375A>G) ACC487GCT (13378A>G 13380C>T) CTT488ACA (13381C>A 13382T>C 13383T>A) GGC490GAC (13388G>A) CGC491CGT (13392C>T) ACC492TTA (13393A>T 13394C>T 13395C>A) TTT493TAT (13397T>A) CGC495CGA (13404C>A) GTC496GTG (13407C>G) ACC498CTT (13411A>C 13412C>T 13413C>T) GGC499GGT (13416C>T) AGC500GGC (13417A>G) TCA501_TGG502insTTTAACGCG (13422_13423insTTTAACGCG) AGC503GGG (13426A>G 13428C>G) GAA504GAG (13431A>G) CGG506CGA (13437G>A) GAA507CAG (13438G>C 13440A>G) GTC508ATT (13441G>A 13443C>T) TAC509TAT (13446C>T) ACC510AAC (13448C>A) TCG511TCA (13452G>A) TAT512AAC (13453T>A 13455T>C) TCA513TCT (13458A>T) CTT514TTG (13459C>T 13461T>G) CCC515CCG (13464C>G) CTG516TTA (13465C>T 13467G>A) AAT517GAG (13468A>G 13470T>G) CTG518TTG (13471C>T) GGC521GGT (13482C>T) GGT522GGC (13485T>C) GCT523GCA (13488T>A) AAG526AAA (13497G>A) ACC534ACA (13521C>A) AGG546CGG (13555A>C) AAT549AAC (13566T>C) GCA570TCA (13627G>T) GTT571GTC (13632T>C) TCC577TCT (13650C>T) ACG579TCG (13654A>T) GGG580GGA (13659G>A) GCG593GCC (13698G>C) CGA596CGC (13707A>C) ACA612ACG (13755A>G) GCA613ACA (13756G>A) CGA615CGT (13764A>T) GTA616GTG (13767A>G) ATC618ATT (13773C>T) ATT619ATC (13776T>C)
139100%27294.8%39 (100%)36 (92.3%)0/0/0/00
Protein mutations:
A3T (16329C>T) E12K (16302C>T) *39C (16219T>A)
Codon mutations:
GCA3ACA (16329C>T) GAG12AAG (16302C>T) TGA39TGT (16219T>A)
1399.8%25698.5%39 (100%)38 (97.4%)0/0/0/00
Protein mutations:none
Codon mutations:none
1181788.0%38788.4%61 (100%)59 (96.7%)0/0/1/00
Protein mutations:none
Codon mutations:
CCG128CMG (27119C>M) GAC132GAT (27132C>T)
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43