NGS Details (SRR3458562_Metagenome_example): Traversvirus tv86

Assembly

1013 (5 contig(s))
18.2
192
12.39 rpm (after QC)
1
de novo + reference guided assembly
Bcf Tools

Coverage Map

Variant Tables

Assignment

Traversvirus tv86 (Taxonomy ID: 2844180)
94.6535
90.625
3
81

Alignment

1785.0 (NT) + 1154.0 (AA) = 2939.0
89.9602
Local, heuristic, nucleotide (BLASTN)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT3539600881.7%178589.3%1010 (99.7%)956 (94.4%)0/3
Mutations:3561C>T, 3563G>A, 3591T>C, 3688T>C, 3691C>T, 3718C>G, 3737delT, 3744G>T, 3776T>G, 5320G>T, 5324G>C, 5326T>G, 5329T>A, 5332C>T, 5394T>A, 47524T>W, 47587A>C, 47588C>A, 57572G>T, 57659A>C, 57661G>C, 57664T>C, 59757A>G, 59761T>G, 59763G>A, 59778A>G, 59784C>T, 59799T>C, 59801A>G, 59817G>A, 59839G>A, 59842A>G, 59843G>T, 59845C>T, 59846A>G, 59847G>A, 59857T>G, 59865G>A, 59870G>A, 59876T>C, 59880G>A, 59888C>T, 59893G>T, 59974_59975delAT, 59998G>A, 60002C>A, 60026C>T, 60034G>A, 60040A>G, 60049T>A, 60066C>T, 60069T>C, 60070A>C, 60081G>T, 60083T>C, 60086G>A
CDS
6056324.4%14493.5%28 (100%)25 (89.3%)0/0/0/01
Protein mutations:A612V (3561C>T), A613T (3563G>A), V622A (3591T>C)
Codon mutations:GCA612GTA (3561C>T), GCG613ACG (3563G>A), GTG622GCG (3591T>C)
13548.6%21592.3%35 (100%)33 (94.3%)0/0/0/00
Protein mutations:Y2D (3776T>G)
Codon mutations:TAC2GAC (3776T>G)
225418.5%23689.1%33 (100%)30 (90.9%)0/0/0/00
Protein mutations:D25Q (5324G>C 5326T>G), I48N (5394T>A)
Codon mutations:GCG23GCT (5320G>T), GAT25CAG (5324G>C 5326T>G), ATT26ATA (5329T>A), CTC27CTT (5332C>T), ATC48AAC (5394T>A)
12415.3%16583.3%24 (100%)22 (91.7%)0/0/1/00
185415.4%21488.4%37 (100%)34 (91.9%)0/0/0/00
Protein mutations:E50D (57659A>C), R51T (57661G>C), I52T (57664T>C)
Codon mutations:GTG21GTT (57572G>T), GAA50GAC (57659A>C), AGA51ACA (57661G>C), ATC52ACC (57664T>C)
175168.6%18084.9%35 (100%)30 (85.7%)0/0/0/02
Protein mutations:S20A (59761T>G 59763G>A), E33G (59801A>G), V46I (59839G>A), S47V (59842A>G 59843G>T), Q48* (59845C>T 59846A>G 59847G>A)
Codon mutations:GCA18GCG (59757A>G), TCG20GCA (59761T>G 59763G>A), GCA25GCG (59778A>G), GGC27GGT (59784C>T), ACT32ACC (59799T>C), GAA33GGA (59801A>G), ACG38ACA (59817G>A), GTC46ATC (59839G>A), AGT47GTT (59842A>G 59843G>T), CAG48TGA (59845C>T 59846A>G 59847G>A)
Proteins
6056324.4%14493.5%28 (100%)25 (89.3%)0/0/0/01
Protein mutations:A612V (3561C>T), A613T (3563G>A), V622A (3591T>C)
Codon mutations:GCA612GTA (3561C>T), GCG613ACG (3563G>A), GTG622GCG (3591T>C)
13548.6%21592.3%35 (100%)33 (94.3%)0/0/0/00
Protein mutations:Y2D (3776T>G)
Codon mutations:TAC2GAC (3776T>G)
225418.5%23689.1%33 (100%)30 (90.9%)0/0/0/00
Protein mutations:D25Q (5324G>C 5326T>G), I48N (5394T>A)
Codon mutations:GCG23GCT (5320G>T), GAT25CAG (5324G>C 5326T>G), ATT26ATA (5329T>A), CTC27CTT (5332C>T), ATC48AAC (5394T>A)
12415.3%16583.3%24 (100%)22 (91.7%)0/0/1/00
Protein mutations:none
Codon mutations:AGC6WGC (47524T>W)
185415.4%21488.4%37 (100%)34 (91.9%)0/0/0/00
Protein mutations:E50D (57659A>C), R51T (57661G>C), I52T (57664T>C)
Codon mutations:GTG21GTT (57572G>T), GAA50GAC (57659A>C), AGA51ACA (57661G>C), ATC52ACC (57664T>C)
175168.6%18084.9%35 (100%)30 (85.7%)0/0/0/02
Protein mutations:S20A (59761T>G 59763G>A), E33G (59801A>G), V46I (59839G>A), S47V (59842A>G 59843G>T), Q48* (59845C>T 59846A>G 59847G>A)
Codon mutations:GCA18GCG (59757A>G), TCG20GCA (59761T>G 59763G>A), GCA25GCG (59778A>G), GGC27GGT (59784C>T), ACT32ACC (59799T>C), GAA33GGA (59801A>G), ACG38ACA (59817G>A), GTC46ATC (59839G>A), AGT47GTT (59842A>G 59843G>T), CAG48TGA (59845C>T 59846A>G 59847G>A)
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43