Version 2.22.2
Genome Detective Platform

NGS Details (SRR3458562_Metagenome_example): Lederbergvirus HK620

Assembly

2625 (5 contig(s))
36.0
850
54.85 rpm (after QC)
1
de novo + reference guided assembly
Bcf Tools

Coverage Map

Variant Tables

Assignment

Lederbergvirus HK620 (Taxonomy ID: 2748764)
94.6667
95.6621
5
58

Alignment

4692.0 (NT) + 5602.0 (AA) = 10294.0
92.5636
Local, heuristic, nucleotide (BLASTN)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT12959296976.9%469289.4%2625 (100%)2485 (94.7%)0/0
Mutations:
12996T>C 17082G>A 17109G>A 17121C>T 17127G>T 17130G>A 17145G>A 17154C>T 17156C>T 17157T>G 17161C>G 17162C>G 17163G>T 17164A>G 17167A>T 17171A>G 17174G>C 26818G>A 26824T>A 26845C>T 26884A>C 26895G>R 26908C>T 26935T>A 27517T>G 27586A>G 27607G>A 27613T>C 27643A>G 27646C>T 27661G>T 27662T>C 27709G>T 27733T>C 27778A>T 27811C>T 27865T>C 27901A>C 27928T>C 27954T>C 27955G>A 27956A>C 27957G>C 27960A>T 27963A>T 27966C>T 27967A>G 27969C>G 28011C>A 28015T>A 28016C>G 28017G>C 28023G>T 28026T>C 28032T>G 28035C>T 28038T>C 28041C>T 28042G>A 28043C>A 28044A>T 28050C>G 28051A>G 28059T>C 28062T>C 28063A>G 28064A>C 28065C>A 28066G>A 28068G>T 28071T>C 28072A>G 28073A>G 28074C>G 28077G>A 28080C>A 28083C>T 28084G>C 28110C>T 28116A>G 28134C>T 28137T>C 28165G>A 28189A>C 28212T>C 28230C>T 28330G>A 28371C>A 28464T>A 28467A>G 28555C>A 28721C>T 28745A>T 28765G>A 28775A>T 28781C>T 28817G>T 28874T>A 28904T>C 28931G>A 28934C>T 28940G>A 28994C>A 29109A>C 29142G>A 29143G>T 29145G>A 29173C>T 29197C>A 29218A>C 29268C>T 29286A>G 29287T>G 29305C>T 29383T>A 29410T>G 29503T>A 29506G>A 29515C>T 29519C>A 29521G>C 29524T>C 29605G>A 29614T>C 29635T>C 29642A>C 29647A>G 29654A>T 29659A>G 29660T>C 29661C>A 29662T>G 29664C>G 29666A>G 29669G>A 29671C>T 29674A>T 29676G>C 29680G>A 29683T>C
CDS
12413710.2%9996.1%14 (100%)14 (100%)0/0/0/01
11931.1%147100%19 (100%)19 (100%)0/0/0/00
7411123.9%20773.4%38 (100%)31 (81.6%)0/0/0/00
Protein mutations:
A104V (17156C>T 17157T>G) P106G (17161C>G 17162C>G 17163G>T) M107V (17164A>G) I108F (17167A>T) D109G (17171A>G) R110P (17174G>C)
Codon mutations:
AAG79AAA (17082G>A) CCG88CCA (17109G>A) GAC92GAT (17121C>T) GTG94GTT (17127G>T) GCG95GCA (17130G>A) AAG100AAA (17145G>A) GGC103GGT (17154C>T) GCT104GTG (17156C>T 17157T>G) CCG106GGT (17161C>G 17162C>G 17163G>T) ATG107GTG (17164A>G) ATT108TTT (17167A>T) GAT109GGT (17171A>G) CGC110CCC (17174G>C)
1141456.8%23994.8%32 (100%)31 (96.9%)0/0/0/00
9847339.1%120794.4%185 (100%)182 (98.4%)0/0/1/02
Protein mutations:
Y334* (27517T>G)
Codon mutations:
CGG101CGA (26818G>A) TCT103TCA (26824T>A) GGC110GGT (26845C>T) ACA123ACC (26884A>C) TGG127TRG (26895G>R) AGC131AGT (26908C>T) TCT140TCA (26935T>A) TAT334TAG (27517T>G) GAA357GAG (27586A>G) CAG364CAA (27607G>A) GAT366GAC (27613T>C) GAA376GAG (27643A>G) CAC377CAT (27646C>T) CCG382CCT (27661G>T) TTG383CTG (27662T>C) TCG398TCT (27709G>T) GCT406GCC (27733T>C) GGA421GGT (27778A>T) TTC432TTT (27811C>T) GTT450GTC (27865T>C) GTA462GTC (27901A>C) ATT471ATC (27928T>C)
1234100%140996.6%234 (100%)222 (94.9%)0/0/0/01
Protein mutations:
E8T (27955G>A 27956A>C 27957G>C) I12V (27967A>G 27969C>G) A37N (28042G>A 28043C>A 28044A>T) T40A (28051A>G) N44A (28063A>G 28064A>C 28065C>A) V45I (28066G>A 28068G>T) N47G (28072A>G 28073A>G 28074C>G) E51Q (28084G>C) A78T (28165G>A) I86L (28189A>C) V133I (28330G>A) L208I (28555C>A)
Codon mutations:
AAT7AAC (27954T>C) GAG8ACC (27955G>A 27956A>C 27957G>C) CCA9CCT (27960A>T) GTA10GTT (27963A>T) ATC11ATT (27966C>T) ATC12GTG (27967A>G 27969C>G) ATC26ATA (28011C>A) TCG28AGC (28015T>A 28016C>G 28017G>C) TCG30TCT (28023G>T) TAT31TAC (28026T>C) CTT33CTG (28032T>G) TAC34TAT (28035C>T) GTT35GTC (28038T>C) ATC36ATT (28041C>T) GCA37AAT (28042G>A 28043C>A 28044A>T) GGC39GGG (28050C>G) ACT40GCT (28051A>G) GTT42GTC (28059T>C) GGT43GGC (28062T>C) AAC44GCA (28063A>G 28064A>C 28065C>A) GTG45ATT (28066G>A 28068G>T) GCT46GCC (28071T>C) AAC47GGG (28072A>G 28073A>G 28074C>G) AAG48AAA (28077G>A) GCC49GCA (28080C>A) AAC50AAT (28083C>T) GAG51CAG (28084G>C) GCC59GCT (28110C>T) GTA61GTG (28116A>G) GAC67GAT (28134C>T) GTT68GTC (28137T>C) GCC78ACC (28165G>A) ATC86CTC (28189A>C) ACT93ACC (28212T>C) GTC99GTT (28230C>T) GTA133ATA (28330G>A) ACC146ACA (28371C>A) ACT177ACA (28464T>A) GCA178GCG (28467A>G) CTC208ATC (28555C>A)
1152100%111099.2%152 (100%)150 (98.7%)0/0/0/01
Protein mutations:
E37D (28745A>T) S44N (28765G>A)
Codon mutations:
GGC29GGT (28721C>T) GAA37GAT (28745A>T) AGC44AAC (28765G>A) GGA47GGT (28775A>T) TTC49TTT (28781C>T) CTG61CTT (28817G>T) ATT80ATA (28874T>A) ACT90ACC (28904T>C) TCG99TCA (28931G>A) TTC100TTT (28934C>T) GCG102GCA (28940G>A) GTC120GTA (28994C>A)
120287.4%118491.1%202 (100%)189 (93.6%)0/0/0/00
Protein mutations:
K6T (29109A>C) W17Y (29142G>A 29143G>T) C18Y (29145G>A) P59L (29268C>T) D65G (29286A>G 29287T>G) Q143N (29519C>A 29521G>C) M188L (29654A>T) S190Q (29660T>C 29661C>A 29662T>G) A191G (29664C>G) I192V (29666A>G) G193S (29669G>A 29671C>T) G195A (29676G>C)
Codon mutations:
AAG6ACG (29109A>C) TGG17TAT (29142G>A 29143G>T) TGC18TAC (29145G>A) AGC27AGT (29173C>T) GCC35GCA (29197C>A) GCA42GCC (29218A>C) CCG59CTG (29268C>T) GAT65GGG (29286A>G 29287T>G) CTC71CTT (29305C>T) GCT97GCA (29383T>A) GCT106GCG (29410T>G) GGT137GGA (29503T>A) CAG138CAA (29506G>A) AAC141AAT (29515C>T) CAG143AAC (29519C>A 29521G>C) AAT144AAC (29524T>C) TCG171TCA (29605G>A) AGT174AGC (29614T>C) AAT181AAC (29635T>C) AGA184CGA (29642A>C) CCA185CCG (29647A>G) ATG188TTG (29654A>T) CAA189CAG (29659A>G) TCT190CAG (29660T>C 29661C>A 29662T>G) GCT191GGT (29664C>G) ATT192GTT (29666A>G) GGC193AGT (29669G>A 29671C>T) GGA194GGT (29674A>T) GGT195GCT (29676G>C) GCG196GCA (29680G>A) TCT197TCC (29683T>C)
Proteins
12413710.2%9996.1%14 (100%)14 (100%)0/0/0/01
Protein mutations:none
Codon mutations:
TAT135TAC (12996T>C)
11931.1%147100%19 (100%)19 (100%)0/0/0/00
Protein mutations:none
Codon mutations:none
7411123.9%20773.4%38 (100%)31 (81.6%)0/0/0/00
Protein mutations:
A104V (17156C>T 17157T>G) P106G (17161C>G 17162C>G 17163G>T) M107V (17164A>G) I108F (17167A>T) D109G (17171A>G) R110P (17174G>C)
Codon mutations:
AAG79AAA (17082G>A) CCG88CCA (17109G>A) GAC92GAT (17121C>T) GTG94GTT (17127G>T) GCG95GCA (17130G>A) AAG100AAA (17145G>A) GGC103GGT (17154C>T) GCT104GTG (17156C>T 17157T>G) CCG106GGT (17161C>G 17162C>G 17163G>T) ATG107GTG (17164A>G) ATT108TTT (17167A>T) GAT109GGT (17171A>G) CGC110CCC (17174G>C)
1141456.8%23994.8%32 (100%)31 (96.9%)0/0/0/00
Protein mutations:none
Codon mutations:none
9847339.1%120794.4%185 (100%)182 (98.4%)0/0/1/02
Protein mutations:
Y334* (27517T>G)
Codon mutations:
CGG101CGA (26818G>A) TCT103TCA (26824T>A) GGC110GGT (26845C>T) ACA123ACC (26884A>C) TGG127TRG (26895G>R) AGC131AGT (26908C>T) TCT140TCA (26935T>A) TAT334TAG (27517T>G) GAA357GAG (27586A>G) CAG364CAA (27607G>A) GAT366GAC (27613T>C) GAA376GAG (27643A>G) CAC377CAT (27646C>T) CCG382CCT (27661G>T) TTG383CTG (27662T>C) TCG398TCT (27709G>T) GCT406GCC (27733T>C) GGA421GGT (27778A>T) TTC432TTT (27811C>T) GTT450GTC (27865T>C) GTA462GTC (27901A>C) ATT471ATC (27928T>C)
1234100%140996.6%234 (100%)222 (94.9%)0/0/0/01
Protein mutations:
E8T (27955G>A 27956A>C 27957G>C) I12V (27967A>G 27969C>G) A37N (28042G>A 28043C>A 28044A>T) T40A (28051A>G) N44A (28063A>G 28064A>C 28065C>A) V45I (28066G>A 28068G>T) N47G (28072A>G 28073A>G 28074C>G) E51Q (28084G>C) A78T (28165G>A) I86L (28189A>C) V133I (28330G>A) L208I (28555C>A)
Codon mutations:
AAT7AAC (27954T>C) GAG8ACC (27955G>A 27956A>C 27957G>C) CCA9CCT (27960A>T) GTA10GTT (27963A>T) ATC11ATT (27966C>T) ATC12GTG (27967A>G 27969C>G) ATC26ATA (28011C>A) TCG28AGC (28015T>A 28016C>G 28017G>C) TCG30TCT (28023G>T) TAT31TAC (28026T>C) CTT33CTG (28032T>G) TAC34TAT (28035C>T) GTT35GTC (28038T>C) ATC36ATT (28041C>T) GCA37AAT (28042G>A 28043C>A 28044A>T) GGC39GGG (28050C>G) ACT40GCT (28051A>G) GTT42GTC (28059T>C) GGT43GGC (28062T>C) AAC44GCA (28063A>G 28064A>C 28065C>A) GTG45ATT (28066G>A 28068G>T) GCT46GCC (28071T>C) AAC47GGG (28072A>G 28073A>G 28074C>G) AAG48AAA (28077G>A) GCC49GCA (28080C>A) AAC50AAT (28083C>T) GAG51CAG (28084G>C) GCC59GCT (28110C>T) GTA61GTG (28116A>G) GAC67GAT (28134C>T) GTT68GTC (28137T>C) GCC78ACC (28165G>A) ATC86CTC (28189A>C) ACT93ACC (28212T>C) GTC99GTT (28230C>T) GTA133ATA (28330G>A) ACC146ACA (28371C>A) ACT177ACA (28464T>A) GCA178GCG (28467A>G) CTC208ATC (28555C>A)
1152100%111099.2%152 (100%)150 (98.7%)0/0/0/01
Protein mutations:
E37D (28745A>T) S44N (28765G>A)
Codon mutations:
GGC29GGT (28721C>T) GAA37GAT (28745A>T) AGC44AAC (28765G>A) GGA47GGT (28775A>T) TTC49TTT (28781C>T) CTG61CTT (28817G>T) ATT80ATA (28874T>A) ACT90ACC (28904T>C) TCG99TCA (28931G>A) TTC100TTT (28934C>T) GCG102GCA (28940G>A) GTC120GTA (28994C>A)
120287.4%118491.1%202 (100%)189 (93.6%)0/0/0/00
Protein mutations:
K6T (29109A>C) W17Y (29142G>A 29143G>T) C18Y (29145G>A) P59L (29268C>T) D65G (29286A>G 29287T>G) Q143N (29519C>A 29521G>C) M188L (29654A>T) S190Q (29660T>C 29661C>A 29662T>G) A191G (29664C>G) I192V (29666A>G) G193S (29669G>A 29671C>T) G195A (29676G>C)
Codon mutations:
AAG6ACG (29109A>C) TGG17TAT (29142G>A 29143G>T) TGC18TAC (29145G>A) AGC27AGT (29173C>T) GCC35GCA (29197C>A) GCA42GCC (29218A>C) CCG59CTG (29268C>T) GAT65GGG (29286A>G 29287T>G) CTC71CTT (29305C>T) GCT97GCA (29383T>A) GCT106GCG (29410T>G) GGT137GGA (29503T>A) CAG138CAA (29506G>A) AAC141AAT (29515C>T) CAG143AAC (29519C>A 29521G>C) AAT144AAC (29524T>C) TCG171TCA (29605G>A) AGT174AGC (29614T>C) AAT181AAC (29635T>C) AGA184CGA (29642A>C) CCA185CCG (29647A>G) ATG188TTG (29654A>T) CAA189CAG (29659A>G) TCT190CAG (29660T>C 29661C>A 29662T>G) GCT191GGT (29664C>G) ATT192GTT (29666A>G) GGC193AGT (29669G>A 29671C>T) GGA194GGT (29674A>T) GGT195GCT (29676G>C) GCG196GCA (29680G>A) TCT197TCC (29683T>C)
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43