Version 2.14.5
Genome Detective Platform

NGS Details (SRR3458562_Metagenome_example): Escherichia phage Lambda_ev243

Assembly

1295 (6 contig(s))
12.4
173
11.16 rpm (after QC)
0
de novo + reference guided assembly
Bcf Tools

Coverage Map

Variant Tables

Assignment

Escherichia phage Lambda_ev243 (Taxonomy ID: 2742957)
96.2162
96.6102
4
68

Alignment

2394.0 (NT) + 2337.0 (AA) = 4731.0
94.9047
Local, heuristic, nucleotide (BLASTN)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT8991436922.8%239492.4%1295 (100%)1246 (96.2%)0/0
Mutations:8996A>T, 9008T>C, 9011G>C, 9014G>C, 9017C>G, 9023C>T, 9024C>G, 9032G>A, 9039C>T, 9041T>G, 9049T>A, 9057T>C, 9058C>A, 9062T>A, 9071A>G, 9074T>G, 9077A>G, 9080C>T, 9089C>G, 9095A>G, 9096C>A, 9101C>G, 9104T>A, 9107G>A, 9110C>A, 22475T>G, 22556T>G, 22558A>G, 22559A>C, 22577T>C, 22625G>T, 36692G>T, 36695C>T, 36698T>C, 36722G>A, 36735G>T, 36782A>T, 36788C>A, 36791G>A, 36797G>A, 41552G>A, 41554T>A, 41565A>G, 41567G>A, 41582A>G, 41628T>G, 41637A>G, 41656T>A, 41657A>T
CDS
155516.6%24986.8%41 (100%)35 (85.4%)0/0/0/00
Protein mutations:K21N (9011G>C), N23K (9017C>G), P26A (9024C>G), V34D (9049T>A), S37Q (9057T>C 9058C>A), L50M (9096C>A)
Codon mutations:ACA16ACT (8996A>T), TAT20TAC (9008T>C), AAG21AAC (9011G>C), GGG22GGC (9014G>C), AAC23AAG (9017C>G), GAC25GAT (9023C>T), CCT26GCT (9024C>G), GCG28GCA (9032G>A), CTT31TTG (9039C>T 9041T>G), GTT34GAT (9049T>A), TCG37CAG (9057T>C 9058C>A), CGT38CGA (9062T>A), AAA41AAG (9071A>G), GTT42GTG (9074T>G), AAA43AAG (9077A>G), GAC44GAT (9080C>T), CCC47CCG (9089C>G), GAA49GAG (9095A>G), CTG50ATG (9096C>A), ACC51ACG (9101C>G), GCT52GCA (9104T>A), GAG53GAA (9107G>A), TCC54TCA (9110C>A)
18522317.5%22692.6%39 (100%)38 (97.4%)0/0/0/01
Protein mutations:Q210K (22625G>T)
Codon mutations:CAA210AAA (22625G>T)
176100%48698.4%76 (100%)74 (97.4%)0/0/0/01
Protein mutations:A54S (36735G>T), E69D (36782A>T)
Codon mutations:GGG39GGT (36692G>T), GTC40GTT (36695C>T), GTT41GTC (36698T>C), GCG49GCA (36722G>A), GCG54TCG (36735G>T), GAA69GAT (36782A>T), ATC71ATA (36788C>A), CAG72CAA (36791G>A), GAG74GAA (36797G>A)
1610671.8%67596.4%89 (100%)88 (98.9%)0/0/0/00
3233456.7%13994.6%23 (100%)22 (95.7%)0/0/0/01
Protein mutations:M340L (41656T>A)
Codon mutations:GGT339GGA (41657A>T), ATG340TTG (41656T>A)
437241.7%17797.8%30 (100%)30 (100%)0/0/0/01
15633.7%38598.7%56 (100%)55 (98.2%)0/0/0/00
Proteins
155516.6%24986.8%41 (100%)35 (85.4%)0/0/0/00
Protein mutations:K21N (9011G>C), N23K (9017C>G), P26A (9024C>G), V34D (9049T>A), S37Q (9057T>C 9058C>A), L50M (9096C>A)
Codon mutations:ACA16ACT (8996A>T), TAT20TAC (9008T>C), AAG21AAC (9011G>C), GGG22GGC (9014G>C), AAC23AAG (9017C>G), GAC25GAT (9023C>T), CCT26GCT (9024C>G), GCG28GCA (9032G>A), CTT31TTG (9039C>T 9041T>G), GTT34GAT (9049T>A), TCG37CAG (9057T>C 9058C>A), CGT38CGA (9062T>A), AAA41AAG (9071A>G), GTT42GTG (9074T>G), AAA43AAG (9077A>G), GAC44GAT (9080C>T), CCC47CCG (9089C>G), GAA49GAG (9095A>G), CTG50ATG (9096C>A), ACC51ACG (9101C>G), GCT52GCA (9104T>A), GAG53GAA (9107G>A), TCC54TCA (9110C>A)
18522317.5%22692.6%39 (100%)38 (97.4%)0/0/0/01
Protein mutations:Q210K (22625G>T)
Codon mutations:CAA210AAA (22625G>T)
176100%48698.4%76 (100%)74 (97.4%)0/0/0/01
Protein mutations:A54S (36735G>T), E69D (36782A>T)
Codon mutations:GGG39GGT (36692G>T), GTC40GTT (36695C>T), GTT41GTC (36698T>C), GCG49GCA (36722G>A), GCG54TCG (36735G>T), GAA69GAT (36782A>T), ATC71ATA (36788C>A), CAG72CAA (36791G>A), GAG74GAA (36797G>A)
1610671.8%67596.4%89 (100%)88 (98.9%)0/0/0/00
Protein mutations:none
Codon mutations:none
3233456.7%13994.6%23 (100%)22 (95.7%)0/0/0/01
Protein mutations:M340L (41656T>A)
Codon mutations:GGT339GGA (41657A>T), ATG340TTG (41656T>A)
437241.7%17797.8%30 (100%)30 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
15633.7%38598.7%56 (100%)55 (98.2%)0/0/0/00
Protein mutations:none
Codon mutations:none
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43