Version 2.22.2
Genome Detective Platform

NGS Details (SRR3458562_Metagenome_example): Escherichia phage Lambda_ev243

Assembly

1295 (6 contig(s))
12.4
173
11.16 rpm (after QC)
0
de novo + reference guided assembly
Bcf Tools

Coverage Map

Variant Tables

Assignment

Escherichia phage Lambda_ev243 (Taxonomy ID: 2742957)
96.2162
96.6102
4
68

Alignment

2394.0 (NT) + 2337.0 (AA) = 4731.0
94.9047
Local, heuristic, nucleotide (BLASTN)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT8991436922.8%239492.4%1295 (100%)1246 (96.2%)0/0
Mutations:
8996A>T 9008T>C 9011G>C 9014G>C 9017C>G 9023C>T 9024C>G 9032G>A 9039C>T 9041T>G 9049T>A 9057T>C 9058C>A 9062T>A 9071A>G 9074T>G 9077A>G 9080C>T 9089C>G 9095A>G 9096C>A 9101C>G 9104T>A 9107G>A 9110C>A 22475T>G 22556T>G 22558A>G 22559A>C 22577T>C 22625G>T 36692G>T 36695C>T 36698T>C 36722G>A 36735G>T 36782A>T 36788C>A 36791G>A 36797G>A 41552G>A 41554T>A 41565A>G 41567G>A 41582A>G 41628T>G 41637A>G 41656T>A 41657A>T
CDS
155516.6%24986.8%41 (100%)35 (85.4%)0/0/0/00
Protein mutations:
K21N (9011G>C) N23K (9017C>G) P26A (9024C>G) V34D (9049T>A) S37Q (9057T>C 9058C>A) L50M (9096C>A)
Codon mutations:
ACA16ACT (8996A>T) TAT20TAC (9008T>C) AAG21AAC (9011G>C) GGG22GGC (9014G>C) AAC23AAG (9017C>G) GAC25GAT (9023C>T) CCT26GCT (9024C>G) GCG28GCA (9032G>A) CTT31TTG (9039C>T 9041T>G) GTT34GAT (9049T>A) TCG37CAG (9057T>C 9058C>A) CGT38CGA (9062T>A) AAA41AAG (9071A>G) GTT42GTG (9074T>G) AAA43AAG (9077A>G) GAC44GAT (9080C>T) CCC47CCG (9089C>G) GAA49GAG (9095A>G) CTG50ATG (9096C>A) ACC51ACG (9101C>G) GCT52GCA (9104T>A) GAG53GAA (9107G>A) TCC54TCA (9110C>A)
18522317.5%22692.6%39 (100%)38 (97.4%)0/0/0/01
Protein mutations:
Q210K (22625G>T)
Codon mutations:
CAA210AAA (22625G>T)
176100%48698.4%76 (100%)74 (97.4%)0/0/0/01
Protein mutations:
A54S (36735G>T) E69D (36782A>T)
Codon mutations:
GGG39GGT (36692G>T) GTC40GTT (36695C>T) GTT41GTC (36698T>C) GCG49GCA (36722G>A) GCG54TCG (36735G>T) GAA69GAT (36782A>T) ATC71ATA (36788C>A) CAG72CAA (36791G>A) GAG74GAA (36797G>A)
1610671.8%67596.4%89 (100%)88 (98.9%)0/0/0/00
3233456.7%13994.6%23 (100%)22 (95.7%)0/0/0/01
Protein mutations:
M340L (41656T>A)
Codon mutations:
GGT339GGA (41657A>T) ATG340TTG (41656T>A)
437241.7%17797.8%30 (100%)30 (100%)0/0/0/01
15633.7%38598.7%56 (100%)55 (98.2%)0/0/0/00
Proteins
155516.6%24986.8%41 (100%)35 (85.4%)0/0/0/00
Protein mutations:
K21N (9011G>C) N23K (9017C>G) P26A (9024C>G) V34D (9049T>A) S37Q (9057T>C 9058C>A) L50M (9096C>A)
Codon mutations:
ACA16ACT (8996A>T) TAT20TAC (9008T>C) AAG21AAC (9011G>C) GGG22GGC (9014G>C) AAC23AAG (9017C>G) GAC25GAT (9023C>T) CCT26GCT (9024C>G) GCG28GCA (9032G>A) CTT31TTG (9039C>T 9041T>G) GTT34GAT (9049T>A) TCG37CAG (9057T>C 9058C>A) CGT38CGA (9062T>A) AAA41AAG (9071A>G) GTT42GTG (9074T>G) AAA43AAG (9077A>G) GAC44GAT (9080C>T) CCC47CCG (9089C>G) GAA49GAG (9095A>G) CTG50ATG (9096C>A) ACC51ACG (9101C>G) GCT52GCA (9104T>A) GAG53GAA (9107G>A) TCC54TCA (9110C>A)
18522317.5%22692.6%39 (100%)38 (97.4%)0/0/0/01
Protein mutations:
Q210K (22625G>T)
Codon mutations:
CAA210AAA (22625G>T)
176100%48698.4%76 (100%)74 (97.4%)0/0/0/01
Protein mutations:
A54S (36735G>T) E69D (36782A>T)
Codon mutations:
GGG39GGT (36692G>T) GTC40GTT (36695C>T) GTT41GTC (36698T>C) GCG49GCA (36722G>A) GCG54TCG (36735G>T) GAA69GAT (36782A>T) ATC71ATA (36788C>A) CAG72CAA (36791G>A) GAG74GAA (36797G>A)
1610671.8%67596.4%89 (100%)88 (98.9%)0/0/0/00
Protein mutations:none
Codon mutations:none
3233456.7%13994.6%23 (100%)22 (95.7%)0/0/0/01
Protein mutations:
M340L (41656T>A)
Codon mutations:
GGT339GGA (41657A>T) ATG340TTG (41656T>A)
437241.7%17797.8%30 (100%)30 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
15633.7%38598.7%56 (100%)55 (98.2%)0/0/0/00
Protein mutations:none
Codon mutations:none
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43