Version 2.14.5
Genome Detective Platform

NGS Details (SRR3458562_Metagenome_example): Escherichia Stx1 converting phage

Assembly

583 (2 contig(s))
28.1
157
10.13 rpm (after QC)
0
de novo + reference guided assembly
Bcf Tools

Coverage Map

Variant Tables

Assignment

Escherichia Stx1 converting phage (Taxonomy ID: 194948)
93.3682
89.1304
2
167

Alignment

975.0 (NT) + 994.0 (AA) = 1969.0
86.4734
Local, heuristic, nucleotide (BLASTN)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT28412529241.0%97586.5%573 (98.3%)535 (91.8%)0/10
Mutations:28421G>A, 28423A>G, 28428A>T, 28434C>T, 28438T>C, 28439T>G, 28451T>C, 28457A>G, 28464G>A, 28465T>A, 28468A>G, 28469C>T, 28491A>G, 28506C>T, 28645C>G, 28648G>T, 28651T>C, 28652T>C, 28653C>G, 28654A>T, 28655G>T, 28659G>A, 28693C>A, 28705G>A, 28732G>T, 28747C>T, 28791T>C, 28793C>G, 28795T>C, 28796T>A, 28797T>C, 28799C>T, 28803A>C, 52753C>T, 52825_52834delCTTATGGACA, 52849A>T, 52859T>G, 52879C>T, 52915T>C
CDS
171100%37786.1%71 (100%)63 (88.7%)0/0/0/01
Protein mutations:L2N (28653C>G 28654A>T 28655G>T), N3G (28651T>C 28652T>C), T4K (28648G>T), C5S (28645C>G), V64T (28468A>G 28469C>T), D65V (28464G>A 28465T>A), S68P (28457A>G), K70E (28451T>C)
Codon mutations:CTG2AAC (28653C>G 28654A>T 28655G>T), AAC3GGC (28651T>C 28652T>C), ACA4AAA (28648G>T), TGT5TCT (28645C>G), GTG51GTA (28506C>T), GGT56GGC (28491A>G), GTA64ACA (28468A>G 28469C>T), GAC65GTT (28464G>A 28465T>A), TCT68CCT (28457A>G), AAG70GAG (28451T>C)
15622.4%31378.3%56 (100%)46 (82.1%)0/0/0/01
Protein mutations:M1I (28648G>T), S3R (28652T>C 28653C>G 28654A>T), A4S (28655G>T), W5* (28659G>A), M20I (28705G>A), L49P (28791T>C), L50V (28793C>G 28795T>C), F51T (28796T>A 28797T>C), P52S (28799C>T), Q53P (28803A>C)
Codon mutations:ATG1ATT (28648G>T), TGT2TGC (28651T>C), TCA3CGT (28652T>C 28653C>G 28654A>T), GCA4TCA (28655G>T), TGG5TAG (28659G>A), GCC16GCA (28693C>A), ATG20ATA (28705G>A), CCG29CCT (28732G>T), CCC34CCT (28747C>T), CTC49CCC (28791T>C), CTT50GTC (28793C>G 28795T>C), TTC51ACC (28796T>A 28797T>C), CCG52TCG (28799C>T), CAA53CCA (28803A>C)
36255.6%30467.7%57 (95.0%)55 (91.7%)0/3/1/10
Protein mutations:Q24P (52859T>G), V33_K35del (52825_52834delCTTATGGACA)
Codon mutations:GGA5GGG (52915T>C), TTG17TTA (52879C>T), CAG24CCG (52859T>G), CCT27CCA (52849A>T), TTT32TT- (52825_52834delCTTATGGACA), GTC33_AAG35del (52825_52834delCTTATGGACA), GAG59GAA (52753C>T)
Proteins
171100%37786.1%71 (100%)63 (88.7%)0/0/0/01
Protein mutations:L2N (28653C>G 28654A>T 28655G>T), N3G (28651T>C 28652T>C), T4K (28648G>T), C5S (28645C>G), V64T (28468A>G 28469C>T), D65V (28464G>A 28465T>A), S68P (28457A>G), K70E (28451T>C)
Codon mutations:CTG2AAC (28653C>G 28654A>T 28655G>T), AAC3GGC (28651T>C 28652T>C), ACA4AAA (28648G>T), TGT5TCT (28645C>G), GTG51GTA (28506C>T), GGT56GGC (28491A>G), GTA64ACA (28468A>G 28469C>T), GAC65GTT (28464G>A 28465T>A), TCT68CCT (28457A>G), AAG70GAG (28451T>C)
15622.4%31378.3%56 (100%)46 (82.1%)0/0/0/01
Protein mutations:M1I (28648G>T), S3R (28652T>C 28653C>G 28654A>T), A4S (28655G>T), W5* (28659G>A), M20I (28705G>A), L49P (28791T>C), L50V (28793C>G 28795T>C), F51T (28796T>A 28797T>C), P52S (28799C>T), Q53P (28803A>C)
Codon mutations:ATG1ATT (28648G>T), TGT2TGC (28651T>C), TCA3CGT (28652T>C 28653C>G 28654A>T), GCA4TCA (28655G>T), TGG5TAG (28659G>A), GCC16GCA (28693C>A), ATG20ATA (28705G>A), CCG29CCT (28732G>T), CCC34CCT (28747C>T), CTC49CCC (28791T>C), CTT50GTC (28793C>G 28795T>C), TTC51ACC (28796T>A 28797T>C), CCG52TCG (28799C>T), CAA53CCA (28803A>C)
36255.6%30467.7%57 (95.0%)55 (91.7%)0/3/1/10
Protein mutations:Q24P (52859T>G), V33_K35del (52825_52834delCTTATGGACA)
Codon mutations:GGA5GGG (52915T>C), TTG17TTA (52879C>T), CAG24CCG (52859T>G), CCT27CCA (52849A>T), TTT32TT- (52825_52834delCTTATGGACA), GTC33_AAG35del (52825_52834delCTTATGGACA), GAG59GAA (52753C>T)
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43