Version 2.22.2
Genome Detective Platform

NGS Details (SRR3458562_Metagenome_example): Enterobacteria phage mEp460

Assembly

624 (4 contig(s))
8.2
57
3.68 rpm (after QC)
0
de novo + reference guided assembly
Bcf Tools

Coverage Map

Variant Tables

Assignment

Enterobacteria phage mEp460 (Taxonomy ID: 1147152)
89.7436
91.2791
2
59

Alignment

992.0 (NT) + 1025.0 (AA) = 2017.0
85.7204
Local, heuristic, nucleotide (BLASTN)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT19201428721.4%99279.5%624 (100%)560 (89.7%)0/0
Mutations:
19211C>T 19232G>A 19247C>T 19254T>C 19256C>T 19271T>C 19273T>C 19274T>C 19278T>C 19283G>T 19289C>T 19292C>T 19298G>T 19316C>T 19322G>C 19330A>G 19335C>A 19340C>A 19344T>A 19384C>T 19388T>A 19412A>G 19414T>A 19431G>T 19433G>A 19435A>G 19442G>C 19448A>G 19451G>T 19460C>T 19466T>A 19472A>G 19475G>A 39699A>T 39702G>A 39704T>C 39723A>G 39730A>G 39733G>A 39748G>A 39749A>G 39750C>T 39758T>A 39794C>T 39817A>G 42294A>T 42300T>G 42309G>A 42312T>C 42314A>G 42318A>G 42322A>C 42323A>G 42324G>T 42333T>G 42785G>A 42788T>A 42797A>G 42800C>A 42810T>C 42815T>G 42863T>C 42865T>A 42866A>C
CDS
401327.7%54789.7%93 (100%)84 (90.3%)0/0/0/00
Protein mutations:
Y57H (19254T>C 19256C>T) I63T (19273T>C 19274T>C) D82G (19330A>G) S87T (19344T>A) A100V (19384C>T) L110Q (19414T>A) A116S (19431G>T 19433G>A) H117R (19435A>G)
Codon mutations:
TCC42TCT (19211C>T) GGG49GGA (19232G>A) GAC54GAT (19247C>T) TAC57CAT (19254T>C 19256C>T) GTT62GTC (19271T>C) ATT63ACC (19273T>C 19274T>C) TTG65CTG (19278T>C) GTG66GTT (19283G>T) GGC68GGT (19289C>T) TTC69TTT (19292C>T) CCG71CCT (19298G>T) ATC77ATT (19316C>T) GTG79GTC (19322G>C) GAT82GGT (19330A>G) CGA84AGA (19335C>A) CCC85CCA (19340C>A) TCG87ACG (19344T>A) GCC100GTC (19384C>T) CGT101CGA (19388T>A) GAA109GAG (19412A>G) CTG110CAG (19414T>A) GCG116TCA (19431G>T 19433G>A) CAT117CGT (19435A>G) GCG119GCC (19442G>C) GCA121GCG (19448A>G) GTG122GTT (19451G>T) AAC125AAT (19460C>T) GCT127GCA (19466T>A) GCA129GCG (19472A>G) AAG130AAA (19475G>A)
2392515.2%6684.6%13 (100%)12 (92.3%)0/0/0/01
Protein mutations:
I243T (39704T>C)
Codon mutations:
GCA241GCT (39699A>T) GAG242GAA (39702G>A) ATA243ACA (39704T>C) ACA249ACG (39723A>G)
759320.4%10691.4%19 (100%)17 (89.5%)0/0/0/01
Protein mutations:
K82R (42314A>G) K85R (42322A>C 42323A>G 42324G>T)
Codon mutations:
ACA75ACT (42294A>T) CTT77CTG (42300T>G) AAG80AAA (42309G>A) ATT81ATC (42312T>C) AAA82AGA (42314A>G) GAA83GAG (42318A>G) AAG85CGT (42322A>C 42323A>G 42324G>T) GCT88GCG (42333T>G)
11514.3%9089.1%15 (100%)14 (93.3%)0/0/0/00
195018.0%21692.3%32 (100%)30 (93.8%)0/0/0/00
Protein mutations:
F30L (42810T>C) I48N (42865T>A 42866A>C)
Codon mutations:
GCG21GCA (42785G>A) TCT22TCA (42788T>A) CAA25CAG (42797A>G) ATC26ATA (42800C>A) TTT30CTT (42810T>C) CTT31CTG (42815T>G) GGT47GGC (42863T>C) ATA48AAC (42865T>A 42866A>C)
Proteins
401327.7%54789.7%93 (100%)84 (90.3%)0/0/0/00
Protein mutations:
Y57H (19254T>C 19256C>T) I63T (19273T>C 19274T>C) D82G (19330A>G) S87T (19344T>A) A100V (19384C>T) L110Q (19414T>A) A116S (19431G>T 19433G>A) H117R (19435A>G)
Codon mutations:
TCC42TCT (19211C>T) GGG49GGA (19232G>A) GAC54GAT (19247C>T) TAC57CAT (19254T>C 19256C>T) GTT62GTC (19271T>C) ATT63ACC (19273T>C 19274T>C) TTG65CTG (19278T>C) GTG66GTT (19283G>T) GGC68GGT (19289C>T) TTC69TTT (19292C>T) CCG71CCT (19298G>T) ATC77ATT (19316C>T) GTG79GTC (19322G>C) GAT82GGT (19330A>G) CGA84AGA (19335C>A) CCC85CCA (19340C>A) TCG87ACG (19344T>A) GCC100GTC (19384C>T) CGT101CGA (19388T>A) GAA109GAG (19412A>G) CTG110CAG (19414T>A) GCG116TCA (19431G>T 19433G>A) CAT117CGT (19435A>G) GCG119GCC (19442G>C) GCA121GCG (19448A>G) GTG122GTT (19451G>T) AAC125AAT (19460C>T) GCT127GCA (19466T>A) GCA129GCG (19472A>G) AAG130AAA (19475G>A)
2392515.2%6684.6%13 (100%)12 (92.3%)0/0/0/01
Protein mutations:
I243T (39704T>C)
Codon mutations:
GCA241GCT (39699A>T) GAG242GAA (39702G>A) ATA243ACA (39704T>C) ACA249ACG (39723A>G)
759320.4%10691.4%19 (100%)17 (89.5%)0/0/0/01
Protein mutations:
K82R (42314A>G) K85R (42322A>C 42323A>G 42324G>T)
Codon mutations:
ACA75ACT (42294A>T) CTT77CTG (42300T>G) AAG80AAA (42309G>A) ATT81ATC (42312T>C) AAA82AGA (42314A>G) GAA83GAG (42318A>G) AAG85CGT (42322A>C 42323A>G 42324G>T) GCT88GCG (42333T>G)
11514.3%9089.1%15 (100%)14 (93.3%)0/0/0/00
Protein mutations:none
Codon mutations:none
195018.0%21692.3%32 (100%)30 (93.8%)0/0/0/00
Protein mutations:
F30L (42810T>C) I48N (42865T>A 42866A>C)
Codon mutations:
GCG21GCA (42785G>A) TCT22TCA (42788T>A) CAA25CAG (42797A>G) ATC26ATA (42800C>A) TTT30CTT (42810T>C) CTT31CTG (42815T>G) GGT47GGC (42863T>C) ATA48AAC (42865T>A 42866A>C)
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43