NGS Details (SRR3458562_Metagenome_example): Peduovirus pro483

Assembly

1132 (6 contig(s))
5.0
51
3.29 rpm (after QC)
1
de novo + reference guided assembly
Bcf Tools

Coverage Map

Variant Tables

Assignment

Peduovirus pro483 (Taxonomy ID: 2560459)
97.0082
97.2973
1
43

Alignment

2038.0 (NT) + 2246.0 (AA) = 4284.0
96.3563
Global, seeded, nucleotide + amino acids (AGA)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT12513234883.9%203894.0%1103 (97.4%)1070 (94.5%)0/29
Mutations:12516A>T, 12517A>T, 12518A>T, 12521T>C, 12565A>G, 12614G>A, 12650C>S, 12659C>T, 12735A>G, 12746C>T, 12755C>T, 12759A>G, 12761G>T, 12836C>T, 12908A>G, 12932G>T, 13019G>A, 14002T>G, 14003C>T, 14006_14034delGTGCAGTTTCCTGACCTGTCACTGCCTGC, 14044T>C, 15942G>T, 15948C>T, 15978C>T, 16026T>C, 16089T>C, 16093G>A, 16127G>T, 16150T>G, 16297C>T, 16312G>A, 23396G>A, 23402C>T, 23487G>A
CDS
7624556.9%89096.6%141 (100%)136 (96.5%)0/0/0/00
Protein mutations:K77F (12516A>T 12517A>T 12518A>T), D93G (12565A>G), T150A (12735A>G), T158A (12759A>G 12761G>T)
Codon mutations:AAA77TTT (12516A>T 12517A>T 12518A>T), GGT78GGC (12521T>C), GAT93GGT (12565A>G), AAG109AAA (12614G>A), ACC121ACS (12650C>S), TAC124TAT (12659C>T), ACG150GCG (12735A>G), CTC153CTT (12746C>T), ACC156ACT (12755C>T), ACG158GCT (12759A>G 12761G>T), GCC183GCT (12836C>T), GAA207GAG (12908A>G), CCG215CCT (12932G>T), AAG244AAA (13019G>A)
197357.4%14451.4%45 (83.3%)44 (81.5%)0/9/1/10
Protein mutations:V54_P62del (14006_14034delGTGCAGTTTCCTGACCTGTCACTGCCTGC)
Codon mutations:GTT52GTG (14002T>G), CTG53TTG (14003C>T), GTG54_CCT62del (14006_14034delGTGCAGTTTCCTGACCTGTCACTGCCTGC), GCG63--G (14006_14034delGTGCAGTTTCCTGACCTGTCACTGCCTGC), GGT66GGC (14044T>C)
11615626.3%29598.7%41 (100%)41 (100%)0/0/0/01
19562.9%59496.7%95 (100%)92 (96.8%)0/0/0/00
Protein mutations:I15T (16089T>C), A28S (16127G>T)
Codon mutations:ATT15ACT (16089T>C), GAG16GAA (16093G>A), GCC28TCC (16127G>T), CGT35CGG (16150T>G), ATC84ATT (16297C>T), GAG89GAA (16312G>A)
28032712.1%32393.9%48 (100%)47 (97.9%)0/0/0/00
Proteins
7624556.9%89096.6%141 (100%)136 (96.5%)0/0/0/00
Protein mutations:K77F (12516A>T 12517A>T 12518A>T), D93G (12565A>G), T150A (12735A>G), T158A (12759A>G 12761G>T)
Codon mutations:AAA77TTT (12516A>T 12517A>T 12518A>T), GGT78GGC (12521T>C), GAT93GGT (12565A>G), AAG109AAA (12614G>A), ACC121ACS (12650C>S), TAC124TAT (12659C>T), ACG150GCG (12735A>G), CTC153CTT (12746C>T), ACC156ACT (12755C>T), ACG158GCT (12759A>G 12761G>T), GCC183GCT (12836C>T), GAA207GAG (12908A>G), CCG215CCT (12932G>T), AAG244AAA (13019G>A)
197357.4%14451.4%45 (83.3%)44 (81.5%)0/9/1/10
Protein mutations:V54_P62del (14006_14034delGTGCAGTTTCCTGACCTGTCACTGCCTGC)
Codon mutations:GTT52GTG (14002T>G), CTG53TTG (14003C>T), GTG54_CCT62del (14006_14034delGTGCAGTTTCCTGACCTGTCACTGCCTGC), GCG63--G (14006_14034delGTGCAGTTTCCTGACCTGTCACTGCCTGC), GGT66GGC (14044T>C)
11615626.3%29598.7%41 (100%)41 (100%)0/0/0/01
Protein mutations:none
Codon mutations:GTG119GTT (15942G>T), GGC121GGT (15948C>T), CCC131CCT (15978C>T), GGT147GGC (16026T>C)
19562.9%59496.7%95 (100%)92 (96.8%)0/0/0/00
Protein mutations:I15T (16089T>C), A28S (16127G>T)
Codon mutations:ATT15ACT (16089T>C), GAG16GAA (16093G>A), GCC28TCC (16127G>T), CGT35CGG (16150T>G), ATC84ATT (16297C>T), GAG89GAA (16312G>A)
28032712.1%32393.9%48 (100%)47 (97.9%)0/0/0/00
Protein mutations:none
Codon mutations:GTG296GTA (23396G>A), GCC298GCT (23402C>T), GCC327AC. (23487G>A)
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43