Version 2.22.2
Genome Detective Platform

NGS Details (SRR3458562_Metagenome_example): Wadgaonvirus wv5004651

Assembly

3584 (11 contig(s))
18.4
655
42.26 rpm (after QC)
0
de novo + reference guided assembly
Bcf Tools

Coverage Map

Variant Tables

Assignment

Wadgaonvirus wv5004651 (Taxonomy ID: 2956672)
96.8489
94.9506
6
57

Alignment

6686.0 (NT) + 5310.0 (AA) = 11996.0
94.0255
Local, heuristic, nucleotide (BLASTN)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT20106382219.1%668693.4%3583 (99.9%)3473 (96.8%)3/1
Mutations:
20108A>T 20110A>C 20117G>A 20120T>C 20123T>C 20147G>A 20153C>A 20155G>A 20159C>G 20181A>G 20182G>A 20186A>G 20191A>T 20207G>A 20236A>T 20254G>T 20259C>G 20272T>C 20281C>T 20287T>C 20295G>A 20305T>G 20314T>A 20317C>T 20328T>C 20329T>G 20383T>C 20406G>A 20416A>G 20425G>A 20429G>C 20440T>A 30434A>C 30459G>A 30460C>G 30467C>T 30472T>C 30473C>A 30476T>A 30651G>A 30655T>G 30662T>G 30666T>C 30723T>C 30900G>A 30945A>G 30957G>A 31264C>T 31297T>C 31305G>A 31516T>C 31521A>G 31528G>T 31534G>A 31540C>T 31552T>C 31555T>C 31558A>T 31574C>T 31585G>C 31591T>C 31597C>T 31654C>T 31672G>A 31673T>G 31684C>G 31696G>T 31705G>A 31714T>C 31788C>T 31832C>T 31904A>C 31912G>T 32567T>C 32597A>G 33241A>T 33250A>G 33329T>C 33371C>T 33380G>A 33407T>C 33488C>T 33500G>A 33518G>A 33756A>G 33758T>C 33759A>T 33761A>T 33763A>T 33794C>T 34981T>A 34996T>C 34997A>T 35172_35173insA 35202A>G 35254_35255insC 35270A>G 35479delA 35497T>C 35617T>G 35656T>C 35671C>T 35719T>C 35740T>C 35746G>C 35746_35747insC 35748G>A 35757G>A 35772T>C 37931A>G 37944A>G 37952C>A 38054G>A 38219G>C
CDS
4875033.4%10289.5%17 (100%)15 (88.2%)0/0/0/01
Protein mutations:
E487D (20108A>T) N488T (20110A>C)
Codon mutations:
GAA487GAT (20108A>T) AAC488ACC (20110A>C) CCG490CCA (20117G>A) CGT491CGC (20120T>C) AAT492AAC (20123T>C) AGG500AGA (20147G>A) GCC502GCA (20153C>A) TGA503TAA (20155G>A)
19748.3%60790.3%97 (100%)88 (90.7%)0/0/0/00
Protein mutations:
H2D (20159C>G) E9G (20181A>G 20182G>A) I11V (20186A>G) V18I (20207G>A) T35S (20259C>G) C47Y (20295G>A) V58A (20328T>C 20329T>G) S84N (20406G>A) E92Q (20429G>C)
Codon mutations:
CAT2GAT (20159C>G) GAG9GGA (20181A>G 20182G>A) ATT11GTT (20186A>G) GCA12GCT (20191A>T) GTT18ATT (20207G>A) ACA27ACT (20236A>T) ACG33ACT (20254G>T) ACT35AGT (20259C>G) GCT39GCC (20272T>C) GTC42GTT (20281C>T) ATT44ATC (20287T>C) TGT47TAT (20295G>A) CTT50CTG (20305T>G) CCT53CCA (20314T>A) GGC54GGT (20317C>T) GTT58GCG (20328T>C 20329T>G) GAT76GAC (20383T>C) AGC84AAC (20406G>A) ACA87ACG (20416A>G) TCG90TCA (20425G>A) GAA92CAA (20429G>C) GCT95GCA (20440T>A)
65691.1%31893.8%51 (100%)48 (94.1%)0/0/0/01
Protein mutations:
L11F (30473C>A) R12G (30472T>C) A16L (30459G>A 30460C>G)
Codon mutations:
CCA10CCT (30476T>A) TTG11TTT (30473C>A) AGG12GGG (30472T>C) CTG13CTA (30467C>T) GCG16CTG (30459G>A 30460C>G) CCT24CCG (30434A>C)
116167.7%70097.2%109 (100%)106 (97.2%)0/0/0/01
Protein mutations:
I154L (30662T>G) K156T (30655T>G)
Codon mutations:
TAC55TAT (30957G>A) CGT59CGC (30945A>G) GCC74GCT (30900G>A) AAA133AAG (30723T>C) GCA152GCG (30666T>C) ATC154CTC (30662T>G) AAA156ACA (30655T>G) AAC157AAT (30651G>A)
115960.4%61396.8%96 (100%)92 (95.8%)0/0/0/01
Protein mutations:
S18N (31305G>A) N90S (31521A>G) E111D (31585G>C) S141A (31673T>G)
Codon mutations:
TTC4TTT (31264C>T) CCT15CCC (31297T>C) AGC18AAC (31305G>A) CTT88CTC (31516T>C) AAT90AGT (31521A>G) GTG92GTT (31528G>T) GGG94GGA (31534G>A) TTC96TTT (31540C>T) ATT100ATC (31552T>C) GGT101GGC (31555T>C) GGA102GGT (31558A>T) CTG108TTG (31574C>T) GAG111GAC (31585G>C) TGT113TGC (31591T>C) ATC115ATT (31597C>T) CGC134CGT (31654C>T) GCG140GCA (31672G>A) TCT141GCT (31673T>G) GTC144GTG (31684C>G) ACG148ACT (31696G>T) GCG151GCA (31705G>A) GTT154GTC (31714T>C)
17173.2%42896.8%71 (100%)69 (97.2%)0/0/0/00
Protein mutations:
S65I (31912G>T)
Codon mutations:
CTG24TTG (31788C>T) GGC38GGT (31832C>T) CTA62CTC (31904A>C) AGT65ATT (31912G>T)
13328.9%20296.2%33 (100%)32 (97.0%)0/0/0/00
11455429.8%101194.7%165 (100%)158 (95.8%)0/0/0/01
Protein mutations:
S154T (33761A>T 33763A>T) L155Q (33758T>C 33759A>T) L156P (33756A>G) Y325H (33250A>G) S328T (33241A>T)
Codon mutations:
AAG143AAA (33794C>T) TCT154ACA (33761A>T 33763A>T) CTA155CAG (33758T>C 33759A>T) CTC156CCC (33756A>G) GCC235GCT (33518G>A) AAC241AAT (33500G>A) CAG245CAA (33488C>T) GAA272GAG (33407T>C) AGC281AGT (33380G>A) CTG284CTA (33371C>T) CTA298CTG (33329T>C) TAT325CAT (33250A>G) TCG328ACG (33241A>T) AAT542AAC (32597A>G) GCA552GCG (32567T>C)
16221.9%41496.5%62 (100%)60 (96.8%)0/0/0/00
Protein mutations:
L53Q (34996T>C 34997A>T)
Codon mutations:
CTA53CAG (34996T>C 34997A>T) GTA58GTT (34981T>A)
160100%25066.7%60 (100%)59 (98.3%)0/0/1/11
111873.4%33853.9%91 (98.9%)86 (93.5%)1/0/2/20
Protein mutations:
V91_R92insX (35746_35747insC) R92H (35748G>A) R95Q (35757G>A) L100P (35772T>C)
Codon mutations:
AAA2AA- (35479delA) CGT8CGC (35497T>C) CGT48CGG (35617T>G) TCT61TCC (35656T>C) GGC66GGT (35671C>T) GGT82GGC (35719T>C) ACT89ACC (35740T>C) GTG91GTC (35746G>C) GTG91_CGC92insC-- (35746_35747insC) CGC92CAC (35748G>A) CGA95CAA (35757G>A) CTA100CCA (35772T>C)
128.0%642.9%2 (100%)1 (50.0%)0/0/0/00
15420.5%32195.5%54 (100%)51 (94.4%)0/0/0/00
Protein mutations:
M33I (37952C>A) I36T (37944A>G)
Codon mutations:
ATG33ATT (37952C>A) ATT36ACT (37944A>G) ATT40ATC (37931A>G)
120.6%00%2 (100%)0 (0%)0/0/0/00
Protein mutations:
M1I (38219G>C)
Codon mutations:
ATG1ATC (38219G>C)
Proteins
4875033.4%10289.5%17 (100%)15 (88.2%)0/0/0/01
Protein mutations:
E487D (20108A>T) N488T (20110A>C)
Codon mutations:
GAA487GAT (20108A>T) AAC488ACC (20110A>C) CCG490CCA (20117G>A) CGT491CGC (20120T>C) AAT492AAC (20123T>C) AGG500AGA (20147G>A) GCC502GCA (20153C>A) TGA503TAA (20155G>A)
19748.3%60790.3%97 (100%)88 (90.7%)0/0/0/00
Protein mutations:
H2D (20159C>G) E9G (20181A>G 20182G>A) I11V (20186A>G) V18I (20207G>A) T35S (20259C>G) C47Y (20295G>A) V58A (20328T>C 20329T>G) S84N (20406G>A) E92Q (20429G>C)
Codon mutations:
CAT2GAT (20159C>G) GAG9GGA (20181A>G 20182G>A) ATT11GTT (20186A>G) GCA12GCT (20191A>T) GTT18ATT (20207G>A) ACA27ACT (20236A>T) ACG33ACT (20254G>T) ACT35AGT (20259C>G) GCT39GCC (20272T>C) GTC42GTT (20281C>T) ATT44ATC (20287T>C) TGT47TAT (20295G>A) CTT50CTG (20305T>G) CCT53CCA (20314T>A) GGC54GGT (20317C>T) GTT58GCG (20328T>C 20329T>G) GAT76GAC (20383T>C) AGC84AAC (20406G>A) ACA87ACG (20416A>G) TCG90TCA (20425G>A) GAA92CAA (20429G>C) GCT95GCA (20440T>A)
65691.1%31893.8%51 (100%)48 (94.1%)0/0/0/01
Protein mutations:
L11F (30473C>A) R12G (30472T>C) A16L (30459G>A 30460C>G)
Codon mutations:
CCA10CCT (30476T>A) TTG11TTT (30473C>A) AGG12GGG (30472T>C) CTG13CTA (30467C>T) GCG16CTG (30459G>A 30460C>G) CCT24CCG (30434A>C)
116167.7%70097.2%109 (100%)106 (97.2%)0/0/0/01
Protein mutations:
I154L (30662T>G) K156T (30655T>G)
Codon mutations:
TAC55TAT (30957G>A) CGT59CGC (30945A>G) GCC74GCT (30900G>A) AAA133AAG (30723T>C) GCA152GCG (30666T>C) ATC154CTC (30662T>G) AAA156ACA (30655T>G) AAC157AAT (30651G>A)
115960.4%61396.8%96 (100%)92 (95.8%)0/0/0/01
Protein mutations:
S18N (31305G>A) N90S (31521A>G) E111D (31585G>C) S141A (31673T>G)
Codon mutations:
TTC4TTT (31264C>T) CCT15CCC (31297T>C) AGC18AAC (31305G>A) CTT88CTC (31516T>C) AAT90AGT (31521A>G) GTG92GTT (31528G>T) GGG94GGA (31534G>A) TTC96TTT (31540C>T) ATT100ATC (31552T>C) GGT101GGC (31555T>C) GGA102GGT (31558A>T) CTG108TTG (31574C>T) GAG111GAC (31585G>C) TGT113TGC (31591T>C) ATC115ATT (31597C>T) CGC134CGT (31654C>T) GCG140GCA (31672G>A) TCT141GCT (31673T>G) GTC144GTG (31684C>G) ACG148ACT (31696G>T) GCG151GCA (31705G>A) GTT154GTC (31714T>C)
17173.2%42896.8%71 (100%)69 (97.2%)0/0/0/00
Protein mutations:
S65I (31912G>T)
Codon mutations:
CTG24TTG (31788C>T) GGC38GGT (31832C>T) CTA62CTC (31904A>C) AGT65ATT (31912G>T)
13328.9%20296.2%33 (100%)32 (97.0%)0/0/0/00
Protein mutations:none
Codon mutations:none
11455429.8%101194.7%165 (100%)158 (95.8%)0/0/0/01
Protein mutations:
S154T (33761A>T 33763A>T) L155Q (33758T>C 33759A>T) L156P (33756A>G) Y325H (33250A>G) S328T (33241A>T)
Codon mutations:
AAG143AAA (33794C>T) TCT154ACA (33761A>T 33763A>T) CTA155CAG (33758T>C 33759A>T) CTC156CCC (33756A>G) GCC235GCT (33518G>A) AAC241AAT (33500G>A) CAG245CAA (33488C>T) GAA272GAG (33407T>C) AGC281AGT (33380G>A) CTG284CTA (33371C>T) CTA298CTG (33329T>C) TAT325CAT (33250A>G) TCG328ACG (33241A>T) AAT542AAC (32597A>G) GCA552GCG (32567T>C)
16221.9%41496.5%62 (100%)60 (96.8%)0/0/0/00
Protein mutations:
L53Q (34996T>C 34997A>T)
Codon mutations:
CTA53CAG (34996T>C 34997A>T) GTA58GTT (34981T>A)
160100%25066.7%60 (100%)59 (98.3%)0/0/1/11
Protein mutations:none
Codon mutations:
ACG59-CG (35479delA)
111873.4%33853.9%91 (98.9%)86 (93.5%)1/0/2/20
Protein mutations:
V91_R92insX (35746_35747insC) R92H (35748G>A) R95Q (35757G>A) L100P (35772T>C)
Codon mutations:
AAA2AA- (35479delA) CGT8CGC (35497T>C) CGT48CGG (35617T>G) TCT61TCC (35656T>C) GGC66GGT (35671C>T) GGT82GGC (35719T>C) ACT89ACC (35740T>C) GTG91GTC (35746G>C) GTG91_CGC92insC-- (35746_35747insC) CGC92CAC (35748G>A) CGA95CAA (35757G>A) CTA100CCA (35772T>C)
128.0%642.9%2 (100%)1 (50.0%)0/0/0/00
Protein mutations:none
Codon mutations:none
15420.5%32195.5%54 (100%)51 (94.4%)0/0/0/00
Protein mutations:
M33I (37952C>A) I36T (37944A>G)
Codon mutations:
ATG33ATT (37952C>A) ATT36ACT (37944A>G) ATT40ATC (37931A>G)
120.6%00%2 (100%)0 (0%)0/0/0/00
Protein mutations:
M1I (38219G>C)
Codon mutations:
ATG1ATC (38219G>C)
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43