NGS Details (SRR3458562_Metagenome_example): Lambdavirus HK630

Assembly

233 (1 contig(s))
7.2
19
1.23 rpm (after QC)
0
de novo + reference guided assembly
Bcf Tools

Coverage Map

Variant Tables

Assignment

Lambdavirus HK630 (Taxonomy ID: 2169969)
84.4828
86.2069
1
69

Alignment

310.0 (NT) + 321.0 (AA) = 631.0
75.9326
Local, heuristic, nucleotide (BLASTN)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT22194224260.5%31068.3%232 (99.6%)196 (84.1%)0/1
Mutations:22195G>C, 22200T>A, 22202G>T, 22203C>T, 22211C>T, 22222G>A, 22225T>G, 22236C>T, 22249T>C, 22266C>G, 22267C>A, 22279A>G, 22282C>A, 22288G>T, 22291G>A, 22300G>A, 22304C>T, 22309C>T, 22351T>A, 22352G>A, 22353T>A, 22354C>T, 22360T>C, 22362T>G, 22372G>A, 22375T>G, 22376T>G, 22378T>A, 22379G>T, 22380delA, 22392A>C, 22397T>C, 22403A>C, 22405A>G, 22406T>G, 22412T>G, 22425A>T
CDS
13819529.7%32183.8%58 (100%)50 (86.2%)0/0/0/01
Protein mutations:V139E (22200T>A), A140F (22202G>T 22203C>T), H143Y (22211C>T), A151V (22236C>T), T161R (22266C>G 22267C>A), K168N (22288G>T), V190N (22352G>A 22353T>A 22354C>T), M193R (22362T>G)
Codon mutations:ATG137.TC (22195G>C), GTA139GAA (22200T>A), GCC140TTC (22202G>T 22203C>T), CAT143TAT (22211C>T), CCG146CCA (22222G>A), CTT147CTG (22225T>G), GCA151GTA (22236C>T), ATT155ATC (22249T>C), ACC161AGA (22266C>G 22267C>A), GAA165GAG (22279A>G), GCC166GCA (22282C>A), AAG168AAT (22288G>T), AAG169AAA (22291G>A), GTG172GTA (22300G>A), CTG174TTG (22304C>T), AAC175AAT (22309C>T), GCT189GCA (22351T>A), GTC190AAT (22352G>A 22353T>A 22354C>T), GTT192GTC (22360T>C), ATG193AGG (22362T>G)
Proteins
13819529.7%32183.8%58 (100%)50 (86.2%)0/0/0/01
Protein mutations:V139E (22200T>A), A140F (22202G>T 22203C>T), H143Y (22211C>T), A151V (22236C>T), T161R (22266C>G 22267C>A), K168N (22288G>T), V190N (22352G>A 22353T>A 22354C>T), M193R (22362T>G)
Codon mutations:ATG137.TC (22195G>C), GTA139GAA (22200T>A), GCC140TTC (22202G>T 22203C>T), CAT143TAT (22211C>T), CCG146CCA (22222G>A), CTT147CTG (22225T>G), GCA151GTA (22236C>T), ATT155ATC (22249T>C), ACC161AGA (22266C>G 22267C>A), GAA165GAG (22279A>G), GCC166GCA (22282C>A), AAG168AAT (22288G>T), AAG169AAA (22291G>A), GTG172GTA (22300G>A), CTG174TTG (22304C>T), AAC175AAT (22309C>T), GCT189GCA (22351T>A), GTC190AAT (22352G>A 22353T>A 22354C>T), GTT192GTC (22360T>C), ATG193AGG (22362T>G)
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43