Genome Detective

NGS Details (SRR3458562_Metagenome_example): Carnivore bocaparvovirus 1

Assembly

Coverage Length

409 (3 contig(s))

Depth Of Coverage

1.0

Number Of Reads

Reads Per Million

0.19 rpm (after QC)

Ambiguities

Assembly Method

de novo + reference guided assembly

Consensus Caller

Bcf Tools

Coverage Map

Map

Variant Tables

Consensus Variants

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Minority Variants

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SNP Variants Table

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Annotated Variants Table

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Assignment

Type

Carnivore bocaparvovirus 1 (Taxonomy ID: 1511866)

Reference Genome

NC_004442.1

NT Identity (%)

97.0588

AA Identity (%)

93.3333

Number Of Stop Codons

Number Of CDS

Alignment

Alignment Score

758.0 (NT) + 1321.0 (AA) = 2079.0

Concordance (%)

95.5417

Alignment Method

Global, seeded, nucleotide + amino acids (AGA)

NT Alignment

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Region Bam File

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CDS Alignment

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Consensus Sequence

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Consensus Contigs

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Genome Region

Sequence starts at position 1711 and ends at position 4339 relative to NC_004442.1 reference sequence.

NC_004442.1

SRR3458562_Metagenome_example-NC_004442.1-74-0

NC_004442.1

SRR3458562_Metagenome_example-NC_004442.1-74-0

NC_004442.1

SRR3458562_Metagenome_example-NC_004442.1-74-0

CTCCATATAAAGACAATATCAAAATGGAGGACGGAAGTGTGATTCTGCTTACTCATAGTTATATAACACACTTATGAATATGCTAATACAGAGATTAGAATATCCAATCATATTGAAACACAAGTACTGCAGTAGTAGATGTGGCTGCTTCTGATTGGATGTTAAGAGTATATAAGGATAGGTATTTCCTGCTTCTGTTCATTTCGCTTCCGGACTTCGTCGAGAGAACATCTCTGACTTCTAACCGTGAGTGATGTCGACGCTTCCAATCGCGTCTTTAGACGACCTTACTCAATTTTTCTACTCCTGCTTATACTTATATCCTGAAATTTCCATATGTGGGGTGGAAGGCTGATGAAGTTCGTCTGCAAAGAGCACTGGGCGGTATTCGGGGAGACGCTCTCGATGATCCATCCGTGTACATCGGGATGCCTGGTCCCGATAGTCCGGTTGAGCAGACGAGTTTTCTCGAGACACATGGACCTGATTCCAGTTATCATCTTAACCTGTCGCATTGTGCACATATGGCTGCCTATAGCGTATTCTCCGTTAAACAGGGAAGAGCACCTCCGGTGGCTAGCATTTTTGTCCAGAGCGAGATTGGCGAACGCGATTTGCACCTTCATTTGGTCCTTGGAGGACAGGGGCTAAATAAATATAGCGCTAAAGCGTTCCGCCATCAGCTACAGTACAAATTCTGTGAAAATCTAATCACCGTTCTAGAGAAACATGTAAACGAAGGAACTACTTCTGATCCTGAAATGTGTTCAGTTCTTATAAAATGTCTATCCAAAGCTAAAAACGAAGAACATGCATATTGTACTATTTTACAATATAAAAGCAGAAGTGGGGACCTCTATGCATGCAGAGTAGATCCAGTAGAATTTATCTGCAACTATCTACTTCGAAAAAACCTTAAATACTACACCTGTATGGATCCAGATAAAGCCACACAACATATTGCCTATTTTGATGCCACAAGTAAGACTTACGCTGTAAC

CTTAGCTAATGGAAAATGGGTTCCAACAGACTGTAGAAAGCAATGGCTAAATATACTTCGCCACAACGTGATCCAAAAAAACGAGCCAATATTTGCCGGAGAAATGTTTGATGTTTTGCCTAAGGTACCTGAATCTTCTTGGAAAATTGATATGCTTTCTTCTCAATCTAAAATAACTAAGAGAGAGACTTTAATGATTGACTGTATGAATCGATGTGAGCAAAATTACTGGTTAACATATGAAGACCTTGTAAATGGGTGCGCCGATCTCGTTGTTATGTTAGAATGTCAATCGGGCGGAAACAAACTTATAGAACAGATACTTAGTATGGTTCATATAAAGATGACGCAAAAATATACTGCGCTTAGTTATATTTTAGCAAAATATGATCCAATCCCATTTACGACTGACAATAAAGTGTGGAAATTATTAACTTTACAGGGATATAATCCTTGGCAATTTGGACACTGGTTATTATGTGTCCTAGATAAAAAGGCGGGAAAGCAGAACACAATAAACTTTTATGGTCCTGCAAGTACCGGGAAAACCAATTTAGCCAAAGCCATTGTAAACGCTGTTCAGTTATATGGATGTGTAAATCACCAAAATAAGAACTTTGTTTTCAATGATTGTACTGCAAAATTAATATGTTGGTGGGAAGAATGTATCATGACTACTGATTGGGTGGAACAAGCAAAATGTATAATGGGGGGCACACAATTTAGAATAGACAGAAAACATAAGGATTCACATCTATTACCGCAAACACCGTTAATAATATCAACCAATCATAATATATATGAAGTCACAGGAGGCAATACCACTACACATGTTCATTCTATGCCACTTCGGGAGAGAGTTGTTCAATTTAACTTTATGAAACAACTTGAAAGCACGTTCGGTGAAATAGAGCCAACAGACGTTGTTGCATTATTACAAGTATGTTACAAAAAATATTCAGTTTCATTGTCAGACTTTCATCGTACTTGGAACATTAAA

AAAACTCCAAATGACTTTCCATTGACGGTTGTCTGTTCAAATCATACACAGGATCTAATTGTTTTCGAGAGTGGTCTATGCGGATCGTGCGGAGGCTATTATCCACTAGAGACGTGGAATGAAAATAACTGCCAAAATATAGAAGAAGAAGACATAACAGGTGAGATCCTTAATCACAATGATATATCCTATGAAGAATTCGAGGAATGGATCACTAACTTCGATTTTTCGCTCTTAGACTCTCCAGAAACACCAAACGCTACCACTCCATCAAGAAAACGAACAAGTTCGGACGAAACCATAAATTCGCCTCCAAAAAAACAACGCAAAACAGAACACAAGGTACTGATCGATTGGTCCTCACAACCATCTACTTCGGCCGAGCAATATGTCTACGAGACATATGAGCAAGAGATCAAAAGCGAGGAGCCGGAGTCGCAGTCCACAGGACTCACGCCGTCCGAATGGGGAGAGAGACTTGGGATCATTCCACAGGGGATGGACCAGGAACCAATCGTCCTCCACTGTTTCGAGACAATCGACTGGGAGGAAGACAACACCGATGCATGTATTCAATGAACATAAAAGCAGAAGCAAAGAACCTCTTCCTGCGTTCTGTGGCTTCTACTGGCACAGCACTAGATTAGCTCGGTGTGGGACTGACTGGATTTTTAATGAGGGAAAACCCAAATTCCAATCAGTATGTCATGAAAACAAAATAACATGGGATCAATGTAGAGAAATGTTATTTGAATTTAAGAAAACAATAGATCAAAAATATAGAAATATTATGTATCATATGGGACGAGGGGGGTTCTGTCAAAAATGTTGTTACTGGGATGATGTGTACACCAAGCATTTGGCTAATGTTAACGATGTTCTCACACAGGATCTGTCTGACGCAGAAATGCTTTCAGCTGCAATGGAGGTAGATGGCTCCTCCGAATAGAAAACCAGGTGGTTGGGTCGTTCCTGGATATAAATATCTTGGACCATTCAA

TCCACTAGATAATGGCACTCCAATAAACAAAGTTGACAAAGCAGCTCAAAAACACGATTTTGCATATCAATCGTATATTAATAAAGGTGAAAATCCATATTTAAACTTCAATAAAGCTGATTCTGATTTTATTGAGGATCTCAAAAACGATTCATCCTTTGCCGGGTGGCTAGGCAAGTCTGCATTTAGACTTAAACGTATTTTGGCTCCACATCTAAAAGAAAACACTCCTCCTGCTAAAAGAGTGGCCGGAAACAGTAGACAAGATAGGGCGCAAAAGAGGAAACTCTATTTTGCGCGTTCTAACAAAAACTCTAAACAAGCTAGGATGGAACCGCAGGAAACTGAGAACACTATTGAGGCTGATGCGGGCATTGCGGGGCGTGCTGGGGGTGGTGGTGGACCTGGTGGAGGTGGATCGGGCGGTGGCTCCGGGGTCGGAGTTAGTACCGGTGGGTGGGAAGGAGGAACGCTGTTCGGAGATAATAGAGTCATCACAGTTAACACACGACAGTGGTATGCACCTATATACAACGGACATAGATACACCAAGCTTGAGGGAACCGGAAACACATTCTGGAAAGGGATAAAAACTCCATGGGGATACTTTAATTTTAACGCGTACGACTCGCACTTTTCACCACAAGACTGGCAACGTCTCACAAATGAGTACAGAAGATGGAGACCAAAAAAAATGATGGTAAAAATATATAACTTACAAATAAAACAGGTAGTTACACTGCAGGGGGACACTTTATATAATAATGATCTTACAGCAGGAGTACACATTTTTTGTGACGGAAGTCATCAATATCCATATTCCCAGCATCCGTGGGATGCTGGAACGATGCCAGAACTTCCCTATAAAGTCTGGTTGCTGGAAAACTATGGTTACTTCCAATTCCAAGGTGACCTAATAGACACGAGCGTGGACGGTGGTTCTCCAGACGTTGAAAATGTAGAAAAGGAAATTGCGAAATCTGCTCCTTTCTACATTCTC

GAAAATGCAAACCATGAGGTGTTACGTACTGGGGAGGAAACAAACTTCCATTTTAACTTTGATTGTGGGTGGGTCAATAATGACCGGGCATACTGCCCACTACAGGCGGACTTTAATCCACTGGTTAAAACCAGACGCTACTTCGCTACACGAAACAACTACAACAACTCAGGAAAATTCGTGTACACGCGATACAGTCCGTATAACAAGCCATCACAGTGGATGCCAGGACCATCGCTTGGATACATTGGAAACACACAAAGCGCGGCTACTCGGGAACAAGCCCTCGGACCAGTAACTGTAGTGACTGCTCCACCTGGGACATCAGCTTACACTGCCTTCACTGAACAACAATCAAAAACCAATCAGCAGTCTGCATCTAATGCAACGTGGTCCGGTTATGATGTCAGTCCAGTAAACTGCGCACGCTCTGGATTCGATAAGATAGGATTAGCCTATGACTCTGCTCCGGAAAGTGAGTTGGAGGAGAAAATATCTATTAGAGACATCGACAACGACATGAGCCGATGGGGTCAAGTGTTCGTGCAAGACGGAACAAACAAGGAAATATCAAACGATAACACTGGTCAAGGGGGAAACACACGACAAAACATGGCAGAGTTAAAAAATGTATGGATGTTCCCCAATCAAGCATGGGATTCGACGCCCATCAGCAGGGACTTCCCGATTTGGGTAAAATCACCAAACACGGACAAACATACACTATTCGATAGCTCGGATGGAACTCTGCCTATGTCGCATCCGCCGGGAACTATATTTGTTAAAGTTGCTAAAATACCAATACCAACTCAGACAAATACAGATTCATATTTAACTCTGTATGTTACCGGTCAGGTCACATGCACAATAGAATGGGAGGTCGAGCGATTTATGACCAAGAACTGGAGACCCGAGTCAAAGAACGACGTATCATCTTTCAGAGACGCCTTCCTATACACAGTGGGAGCAGACGGAACTTATAATACACCAGAAAGGTTCC

TGGAAGGAATGCCAACAAGAAGAGGCATAAACAAAACGCTGTAATTGATATAATCTTTACCATGTAGTAGTATACCGTCACCACTGGGTGTGTTTTA

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......................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................GGGGCACACAATTTAGAATAGACAGAAAACATAAAGATTCACATCTATTACCACAGACGCCCTTAATCATATCAACCAATCACAATATATATGAAGTCACAGGAGGCAATACCACTACACATGTTCATTCTATGCC..........................................................................................................................................................

.......................................................................................................................................................................................................................................................................................................................................................................................................................................................................GGTGGGAAGGAGGAACGCTGTTCGGAGATAATAGAGTCATCACAGTTAACACACGACAGTGGTATGCACCTATATACAACGGACATAGATACACCAAGCTTGAGGGAACCGGAAACACATTCTGGAAAGGGATAAA.........................................................................................................................................................................................................................................................................................................................................................................................................................

..........................................................................................................................................................................................................ATAACCAGCCATCACAGTGGATG-CAGGACCATCGATTGGATACAGTGGAAACACACAAAGCGCGGCTACTCCGGAACAAGCCCTCGGACCAGTAACTGTAGTGACTGCTCCACCTGGGACATCAGCTTACAATGCC.....................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................................

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Mvocgp1

MPGPDSPVEQTSFLETHGPDSSYHLNLSHCAHMAAYSVFSVKQGRAPPVASIFVQSEIGERDLHLHLVLGGQGLNKYSAKAFRHQLQYKFCENLITVLEKHVNEGTTSDPEMCSVLIKCLSKAKNEEHAYCTILQYKSRSGDLYACRVDPVEFICNYLLRKNLKYYTCMDPDKATQHIAYFDATSKTYAVTLANGKWVPTDCRKQWLNILRHNVIQKNEPIFAGEMFDVLPKVPESSWKIDMLSSQSKITKRETLMIDCMNRCEQNYWLTYEDLVNGCADLVVMLECQSGGNKLIEQILSMVHIKMTQKYTALSYILAKYDPIPFTTDNKVWKLLTLQGYNPWQFGHWLLCVLDKKAGKQNTINFYGPASTGKTNLAKAIVNAVQLYGCVNHQNKNFVFNDCTAKLICWWEECIMTTDWVEQAKCIMGGTQFRIDRKHKDSHLLPQTPLIISTNHNIYEVTGGNTTTHVHSMPLRERVVQFNFMKQLESTFGEIEPTDVVALLQVCYKKYSVSLSDFHRTWNIKKTPNDFPLTVVCSNHTQDLIVFESGLCGSCGGYYPLETWNENNCQNIEEEDITGEILNHNDISYEEFEEWITNFDFSLLDSPETPNATTPSRKRTSSDETINSPPKKQRKTEHKVLIDWSSQPSTSAEQYVYETYEQEIKSEEPESQSTGLTPSEWGERLGIIPQGMDQEPIVLHCFETIDWEEDNTDACIQ*

...........................................................................................................................................................................................................................................................................................................................................................................................................................................XGTQFRIDRKHKDSHLLPQTPLIISTNHNIYEVTGGNTTTHVHSMX....................................................................................................................................................................................................................................................

Mvocgp2

MSTRHMSKRSKARSRSRSPQDSRRPNGERDLGSFHRGWTRNQSSSTVSRQSTGRKTTPMHVFNEHKSRSKEPLPAFCGFYWHSTRLARCGTDWIFNEGKPKFQSVCHENKITWDQCREMLFEFKKTIDQKYRNIMYHMGRGGFCQKCCYWDDVYTKHLANVNDVLTQDLSDAEMLSAAMEVDGSSE*

...........................................................................................................................................................................................

Mvocgp3

MAPPNRKPGGWVVPGYKYLGPFNPLDNGTPINKVDKAAQKHDFAYQSYINKGENPYLNFNKADSDFIEDLKNDSSFAGWLGKSAFRLKRILAPHLKENTPPAKRVAGNSRQDRAQKRKLYFARSNKNSKQARMEPQETENTIEADAGIAGRAGGGGGPGGGGSGGGSGVGVSTGGWEGGTLFGDNRVITVNTRQWYAPIYNGHRYTKLEGTGNTFWKGIKTPWGYFNFNAYDSHFSPQDWQRLTNEYRRWRPKKMMVKIYNLQIKQVVTLQGDTLYNNDLTAGVHIFCDGSHQYPYSQHPWDAGTMPELPYKVWLLENYGYFQFQGDLIDTSVDGGSPDVENVEKEIAKSAPFYILENANHEVLRTGEETNFHFNFDCGWVNNDRAYCPLQADFNPLVKTRRYFATRNNYNNSGKFVYTRYSPYNKPSQWMPGPSLGYIGNTQSAATREQALGPVTVVTAPPGTSAYTAFTEQQSKTNQQSASNATWSGYDVSPVNCARSGFDKIGLAYDSAPESELEEKISIRDIDNDMSRWGQVFVQDGTNKEISNDNTGQGGNTRQNMAELKNVWMFPNQAWDSTPISRDFPIWVKSPNTDKHTLFDSSDGTLPMSHPPGTIFVKVAKIPIPTQTNTDSYLTLYVTGQVTCTIEWEVERFMTKNWRPESKNDVSSFRDAFLYTVGADGTYNTPERFLEGMPTRRGINKTL*

..............................................................................................................................................................................XWEGGTLFGDNRVITVNTRQWYAPIYNGHRYTKLEGTGNTFWKGIX...........................................................................................................................................................................................................XNQPSQWMXGPSIGYSGNTQSAATPEQALGPVTVVTAPPGTSAYNA...........................................................................................................................................................................................................................................

Alignment Detailed Statistics


	Begin	End	Coverage	Score	Concordance	Matches	Identities	I/D/M/F^*	Stop Codons
NT	1711	4339	8.0%	758	94.0%	408 (99.8%)	396 (96.8%)	0/1
Mutations:	1745G>A, 1763G>A, 1766A>G, 1769A>G, 1772G>C, 1778A>C, 1793T>C, 4208A>C, 4226delC, 4238C>A, 4248T>G, 4275G>C, 4335C>A
CDS

Mvocgp1	429	473	6.3%	321	94.1%	45 (100%)	44 (97.8%)	0/0/0/0	0
Mvocgp3	176	469	12.8%	500	73.6%	90 (100%)	83 (92.2%)	0/0/1/1	0
Protein mutations:	K426Q (4208A>C), L436I (4238C>A), I439S (4248T>G), R448P (4275G>C), T468N (4335C>A)
Codon mutations:	AAG426CAG (4208A>C), CCA432-CA (4226delC), CTT436ATT (4238C>A), ATT439AGT (4248T>G), CGG448CCG (4275G>C), ACT468AAT (4335C>A)
Mvocgp4	44	337	15.7%	500	73.6%	90 (100%)	83 (92.2%)	0/0/1/1	0
Protein mutations:	K294Q (4208A>C), L304I (4238C>A), I307S (4248T>G), R316P (4275G>C), T336N (4335C>A)
Codon mutations:	AAG294CAG (4208A>C), CCA300-CA (4226delC), CTT304ATT (4238C>A), ATT307AGT (4248T>G), CGG316CCG (4275G>C), ACT336AAT (4335C>A)
Proteins

non structural protein 1 (NP_758521.1)	429	473	6.3%	321	94.1%	45 (100%)	44 (97.8%)	0/0/0/0	0
Protein mutations:	none
Codon mutations:	AAG439AAA (1745G>A), CCG445CCA (1763G>A), CAA446CAG (1766A>G), ACA447ACG (1769A>G), CCG448CCC (1772G>C), ATA450ATC (1778A>C), CAT455CAC (1793T>C)
virus protein 1 (NP_758523.1)	176	469	12.8%	500	73.6%	90 (100%)	83 (92.2%)	0/0/1/1	0
Protein mutations:	K426Q (4208A>C), L436I (4238C>A), I439S (4248T>G), R448P (4275G>C), T468N (4335C>A)
Codon mutations:	AAG426CAG (4208A>C), CCA432-CA (4226delC), CTT436ATT (4238C>A), ATT439AGT (4248T>G), CGG448CCG (4275G>C), ACT468AAT (4335C>A)
virus protein 2 (NP_758524.1)	44	337	15.7%	500	73.6%	90 (100%)	83 (92.2%)	0/0/1/1	0
Protein mutations:	K294Q (4208A>C), L304I (4238C>A), I307S (4248T>G), R316P (4275G>C), T336N (4335C>A)
Codon mutations:	AAG294CAG (4208A>C), CCA300-CA (4226delC), CTT304ATT (4238C>A), ATT307AGT (4248T>G), CGG316CCG (4275G>C), ACT336AAT (4335C>A)

^*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43