NGS Details (SRR3458562_Metagenome_example): Feline leukemia virus

Assembly

139 (1 contig(s))
1.5
2
0.13 rpm (after QC)
0
de novo + reference guided assembly
Bcf Tools

Coverage Map

Variant Tables

Assignment

Feline leukemia virus (Taxonomy ID: 11768)
80.8824
76.087
0
2

Alignment

156.0 (NT) + 259.0 (AA) = 415.0
73.4513
Global, seeded, nucleotide + amino acids (AGA)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT539255301.6%15660.0%136 (97.8%)110 (79.1%)0/3
Mutations:5395C>A, 5400G>T, 5404A>G, 5405T>C, 5407A>G, 5415G>A, 5442T>C, 5448T>A, 5457A>G, 5464A>G, 5466T>C, 5470T>A, 5471C>T, 5473A>T, 5475T>A, 5484T>C, 5485T>C, 5490T>C, 5496A>G, 5499G>C, 5500C>A, 5502A>G, 5505A>G, 5512T>A, 5515_5517delAAG, 5518G>A, 5527C>G
CDS
157016162.6%25983.8%46 (97.9%)35 (74.5%)0/1/0/00
Protein mutations:L1571I (5395C>A), I1574A (5404A>G 5405T>C), N1575D (5407A>G), N1594D (5464A>G 5466T>C), S1596I (5470T>A 5471C>T), I1597L (5473A>T 5475T>A), L1606M (5500C>A 5502A>G), S1610T (5512T>A), K1611del (5515_5517delAAG), D1612N (5518G>A), L1615V (5527C>G)
Codon mutations:CTA1571ATA (5395C>A), CTG1572CTT (5400G>T), ATT1574GCT (5404A>G 5405T>C), AAT1575GAT (5407A>G), AAG1577AAA (5415G>A), AGT1586AGC (5442T>C), GGT1588GGA (5448T>A), GAA1591GAG (5457A>G), AAT1594GAC (5464A>G 5466T>C), TCA1596ATA (5470T>A 5471C>T), ATT1597TTA (5473A>T 5475T>A), ACT1600ACC (5484T>C), TTA1601CTA (5485T>C), ACT1602ACC (5490T>C), TTA1604TTG (5496A>G), ACG1605ACC (5499G>C), CTA1606ATG (5500C>A 5502A>G), GAA1607GAG (5505A>G), TCT1610ACT (5512T>A), AAG1611del (5515_5517delAAG), GAT1612AAT (5518G>A), CTC1615GTC (5527C>G)
Proteins
157016162.6%25983.8%46 (97.9%)35 (74.5%)0/1/0/00
Protein mutations:L1571I (5395C>A), I1574A (5404A>G 5405T>C), N1575D (5407A>G), N1594D (5464A>G 5466T>C), S1596I (5470T>A 5471C>T), I1597L (5473A>T 5475T>A), L1606M (5500C>A 5502A>G), S1610T (5512T>A), K1611del (5515_5517delAAG), D1612N (5518G>A), L1615V (5527C>G)
Codon mutations:CTA1571ATA (5395C>A), CTG1572CTT (5400G>T), ATT1574GCT (5404A>G 5405T>C), AAT1575GAT (5407A>G), AAG1577AAA (5415G>A), AGT1586AGC (5442T>C), GGT1588GGA (5448T>A), GAA1591GAG (5457A>G), AAT1594GAC (5464A>G 5466T>C), TCA1596ATA (5470T>A 5471C>T), ATT1597TTA (5473A>T 5475T>A), ACT1600ACC (5484T>C), TTA1601CTA (5485T>C), ACT1602ACC (5490T>C), TTA1604TTG (5496A>G), ACG1605ACC (5499G>C), CTA1606ATG (5500C>A 5502A>G), GAA1607GAG (5505A>G), TCT1610ACT (5512T>A), AAG1611del (5515_5517delAAG), GAT1612AAT (5518G>A), CTC1615GTC (5527C>G)
99410403.9%25983.8%46 (97.9%)35 (74.5%)0/1/0/00
Protein mutations:L995I (5395C>A), I998A (5404A>G 5405T>C), N999D (5407A>G), N1018D (5464A>G 5466T>C), S1020I (5470T>A 5471C>T), I1021L (5473A>T 5475T>A), L1030M (5500C>A 5502A>G), S1034T (5512T>A), K1035del (5515_5517delAAG), D1036N (5518G>A), L1039V (5527C>G)
Codon mutations:CTA995ATA (5395C>A), CTG996CTT (5400G>T), ATT998GCT (5404A>G 5405T>C), AAT999GAT (5407A>G), AAG1001AAA (5415G>A), AGT1010AGC (5442T>C), GGT1012GGA (5448T>A), GAA1015GAG (5457A>G), AAT1018GAC (5464A>G 5466T>C), TCA1020ATA (5470T>A 5471C>T), ATT1021TTA (5473A>T 5475T>A), ACT1024ACC (5484T>C), TTA1025CTA (5485T>C), ACT1026ACC (5490T>C), TTA1028TTG (5496A>G), ACG1029ACC (5499G>C), CTA1030ATG (5500C>A 5502A>G), GAA1031GAG (5505A>G), TCT1034ACT (5512T>A), AAG1035del (5515_5517delAAG), GAT1036AAT (5518G>A), CTC1039GTC (5527C>G)
20024611.3%25983.8%46 (97.9%)35 (74.5%)0/1/0/00
Protein mutations:L201I (5395C>A), I204A (5404A>G 5405T>C), N205D (5407A>G), N224D (5464A>G 5466T>C), S226I (5470T>A 5471C>T), I227L (5473A>T 5475T>A), L236M (5500C>A 5502A>G), S240T (5512T>A), K241del (5515_5517delAAG), D242N (5518G>A), L245V (5527C>G)
Codon mutations:CTA201ATA (5395C>A), CTG202CTT (5400G>T), ATT204GCT (5404A>G 5405T>C), AAT205GAT (5407A>G), AAG207AAA (5415G>A), AGT216AGC (5442T>C), GGT218GGA (5448T>A), GAA221GAG (5457A>G), AAT224GAC (5464A>G 5466T>C), TCA226ATA (5470T>A 5471C>T), ATT227TTA (5473A>T 5475T>A), ACT230ACC (5484T>C), TTA231CTA (5485T>C), ACT232ACC (5490T>C), TTA234TTG (5496A>G), ACG235ACC (5499G>C), CTA236ATG (5500C>A 5502A>G), GAA237GAG (5505A>G), TCT240ACT (5512T>A), AAG241del (5515_5517delAAG), GAT242AAT (5518G>A), CTC245GTC (5527C>G)
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43