Version 2.22.2
Genome Detective Platform

NGS Details (SRR3458562_Metagenome_example): Escherichia phage Lambda_ev099

Assembly

2403 (11 contig(s))
12.8
325
20.97 rpm (after QC)
3
de novo + reference guided assembly
Bcf Tools

Coverage Map

Variant Tables

Assignment

Escherichia phage Lambda_ev099 (Taxonomy ID: 2742953)
95.0417
91.3158
3
73

Alignment

4318.0 (NT) + 4807.0 (AA) = 9125.0
90.6967
Local, heuristic, nucleotide (BLASTN)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT20375471805.1%431890.2%2400 (99.9%)2281 (94.9%)0/3
Mutations:
20399T>C 20417T>A 20422T>C 20423C>T 20429C>A 20481T>G 20495T>C 20531C>T 20576G>A 20600A>G 20611G>A 20630G>A 20632C>T 20633G>A 20708C>T 20711C>T 20717A>G 20720C>T 20728C>T 20984T>A 20997G>A 20999A>G 21008A>T 21026A>G 21035A>G 21065T>G 21123G>A 21146C>T 21150A>G 21152T>C 21155G>T 21158T>C 21161A>G 21162A>T 21164G>A 22617T>G 22625A>K 22626C>Y 22627A>W 22690C>A 22955C>T 22958C>T 22964T>A 22970C>T 22971G>T 22973A>T 22994C>A 23006T>G 23030C>T 23063G>T 23147C>G 23263G>A 23275G>T 23320T>C 26204T>C 26211T>C 26231A>G 26249A>G 26265A>G 26300A>G 26303G>C 26310T>C 26327G>C 26344A>G 26349G>C 26358C>T 26385G>A 26388T>G 26391G>T 26400T>G 26415G>A 26417A>G 26439A>C 26442C>G 26444G>C 28330G>T 28385G>A 41492G>A 44417A>G 44424C>T 44555C>T 44556G>A 44558T>G 44560G>C 44564G>A 44862C>T 44877A>G 44880C>A 44892A>G 44900G>C 44901A>G 44902C>T 44911A>T 44921C>T 44945C>T 44964G>A 44966C>T 44972A>T 45014C>T 45032C>T 45037A>C 45055G>A 46802T>C 46854A>C 46933T>A 46987A>G 47013_47015delCGT 47016T>C 47029T>C 47032C>T 47038T>C 47080A>G 47102T>C 47145G>C 47147A>C 47148G>C 47150T>C 47162C>T 47166C>T 47177T>C
CDS
11623.5%8293.2%16 (100%)15 (93.8%)0/0/0/00
Protein mutations:
M1V (20422T>C)
Codon mutations:
ATG1GTG (20422T>C) GCA2GCT (20417T>A) GTA8GTG (20399T>C)
122868.9%106085.8%157 (100%)135 (86.0%)0/0/0/00
Protein mutations:
H6P (21065T>G) L16P (21035A>G) L19P (21026A>G) V25E (21008A>T) L28P (20999A>G) P29S (20997G>A) Q33L (20984T>A) R121Q (20720C>T) M122T (20717A>G) R124Q (20711C>T) R125H (20708C>T) T150I (20632C>T 20633G>A) T151M (20630G>A) L161P (20600A>G) S169F (20576G>A) R184Q (20531C>T) Y196C (20495T>C) M201L (20481T>G) G218V (20429C>A) R220K (20422T>C 20423C>T) Q222L (20417T>A) *228W (20399T>C)
Codon mutations:
CAC6CCC (21065T>G) CTG16CCG (21035A>G) CTG19CCG (21026A>G) GTG25GAG (21008A>T) CTG28CCG (20999A>G) CCG29TCG (20997G>A) CAG33CTG (20984T>A) CTG118CTA (20728C>T) CGG121CAG (20720C>T) ATG122ACG (20717A>G) CGG124CAG (20711C>T) CGC125CAC (20708C>T) ACG150ATA (20632C>T 20633G>A) ACG151ATG (20630G>A) AGC157AGT (20611G>A) CTG161CCG (20600A>G) TCT169TTT (20576G>A) CGG184CAG (20531C>T) TAC196TGC (20495T>C) ATG201CTG (20481T>G) GGG218GTG (20429C>A) AGA220AAG (20422T>C 20423C>T) CAG222CTG (20417T>A) TAG228TGG (20399T>C)
12810.4%12991.5%28 (100%)24 (85.7%)0/0/0/00
Protein mutations:
V13I (21123G>A) S22G (21150A>G 21152T>C) T26S (21162A>T 21164G>A)
Codon mutations:
GTA13ATA (21123G>A) ACC20ACT (21146C>T) AGT22GGC (21150A>G 21152T>C) ACG23ACT (21155G>T) TCT24TCC (21158T>C) GAA25GAG (21161A>G) ACG26TCA (21162A>T 21164G>A)
24141125.5%60587.9%105 (100%)99 (94.3%)0/0/2/01
Protein mutations:
S243A (22617T>G) T267N (22690C>A) A361S (22971G>T 22973A>T)
Codon mutations:
TCT243GCT (22617T>G) GAA245GAK (22625A>K) CAG246YWG (22626C>Y 22627A>W) ACC267AAC (22690C>A) TCC355TCT (22955C>T) GGC356GGT (22958C>T) GCT358GCA (22964T>A) AGC360AGT (22970C>T) GCA361TCT (22971G>T 22973A>T) GTC368GTA (22994C>A) GCT372GCG (23006T>G) GGC380GGT (23030C>T) GCG391GCT (23063G>T)
18543.8%59398.0%85 (100%)84 (98.8%)0/0/0/00
Protein mutations:
P9A (23147C>G)
Codon mutations:
CCA9GCA (23147C>G) CCG47CCA (23263G>A) CCG51CCT (23275G>T) CAT66CAC (23320T>C)
15546.6%34185.7%55 (100%)48 (87.3%)0/0/0/00
Protein mutations:
L2S (26344A>G) R8G (26327G>C) Q16E (26303G>C) S17P (26300A>G) W34R (26249A>G) I49V (26204T>C)
Codon mutations:
TTA2TCA (26344A>G) CGC8GGC (26327G>C) GTA13GTG (26310T>C) CAG16GAG (26303G>C) TCC17CCC (26300A>G) TAT28TAC (26265A>G) TGG34CGG (26249A>G) TTG40CTG (26231A>G) ACA46ACG (26211T>C) ATC49GTC (26204T>C)
325644.6%16896.6%25 (100%)25 (100%)0/0/0/01
173613.6%13190.3%20 (100%)19 (95.0%)0/0/0/00
477524.0%218100%29 (100%)29 (100%)0/0/0/00
358530.7%31687.5%51 (100%)45 (88.2%)0/0/0/00
Protein mutations:
E35G (44417A>G) T81I (44555C>T 44556G>A) V82G (44558T>G) A83P (44560G>C) R84K (44564G>A)
Codon mutations:
GAA35GGA (44417A>G) GTC37GTT (44424C>T) ACG81ATA (44555C>T 44556G>A) GTC82GGC (44558T>G) GCC83CCC (44560G>C) AGA84AAA (44564G>A)
178446.6%37582.6%68 (100%)59 (86.8%)0/0/0/00
Protein mutations:
N24D (44877A>G) H25N (44880C>A) N29D (44892A>G) M31I (44900G>C) T32V (44901A>G 44902C>T) E35V (44911A>T) D53N (44964G>A 44966C>T) D77A (45037A>C) R83H (45055G>A)
Codon mutations:
CTG19TTG (44862C>T) AAT24GAT (44877A>G) CAT25AAT (44880C>A) AAC29GAC (44892A>G) ATG31ATC (44900G>C) ACC32GTC (44901A>G 44902C>T) GAA35GTA (44911A>T) GAC38GAT (44921C>T) GAC46GAT (44945C>T) GAC53AAT (44964G>A 44966C>T) GCA55GCT (44972A>T) AAC69AAT (45014C>T) CTC75CTT (45032C>T) GAT77GCT (45037A>C) CGT83CAT (45055G>A)
17494.9%47196.1%73 (98.6%)70 (94.6%)0/1/0/00
Protein mutations:
T3del (47013_47015delCGT) I12T (46987A>G) D30V (46933T>A) I74V (46802T>C)
Codon mutations:
TCA2TCG (47016T>C) ACG3del (47013_47015delCGT) ATC12ACC (46987A>G) GAT30GTT (46933T>A) TCT56TCG (46854A>C) ATC74GTC (46802T>C)
156277.4%31891.4%48 (100%)42 (87.5%)0/0/0/01
Protein mutations:
N16D (47177T>C) V21I (47162C>T) I25V (47148G>C 47150T>C) Y26E (47145G>C 47147A>C) I41V (47102T>C) L48P (47080A>G)
Codon mutations:
AAT16GAT (47177T>C) CTG19CTA (47166C>T) GTC21ATC (47162C>T) ATC25GTG (47148G>C 47150T>C) TAC26GAG (47145G>C 47147A>C) ATA41GTA (47102T>C) CTT48CCT (47080A>G) TAA62TGA (47038T>C)
Proteins
11623.5%8293.2%16 (100%)15 (93.8%)0/0/0/00
Protein mutations:
M1V (20422T>C)
Codon mutations:
ATG1GTG (20422T>C) GCA2GCT (20417T>A) GTA8GTG (20399T>C)
122868.9%106085.8%157 (100%)135 (86.0%)0/0/0/00
Protein mutations:
H6P (21065T>G) L16P (21035A>G) L19P (21026A>G) V25E (21008A>T) L28P (20999A>G) P29S (20997G>A) Q33L (20984T>A) R121Q (20720C>T) M122T (20717A>G) R124Q (20711C>T) R125H (20708C>T) T150I (20632C>T 20633G>A) T151M (20630G>A) L161P (20600A>G) S169F (20576G>A) R184Q (20531C>T) Y196C (20495T>C) M201L (20481T>G) G218V (20429C>A) R220K (20422T>C 20423C>T) Q222L (20417T>A) *228W (20399T>C)
Codon mutations:
CAC6CCC (21065T>G) CTG16CCG (21035A>G) CTG19CCG (21026A>G) GTG25GAG (21008A>T) CTG28CCG (20999A>G) CCG29TCG (20997G>A) CAG33CTG (20984T>A) CTG118CTA (20728C>T) CGG121CAG (20720C>T) ATG122ACG (20717A>G) CGG124CAG (20711C>T) CGC125CAC (20708C>T) ACG150ATA (20632C>T 20633G>A) ACG151ATG (20630G>A) AGC157AGT (20611G>A) CTG161CCG (20600A>G) TCT169TTT (20576G>A) CGG184CAG (20531C>T) TAC196TGC (20495T>C) ATG201CTG (20481T>G) GGG218GTG (20429C>A) AGA220AAG (20422T>C 20423C>T) CAG222CTG (20417T>A) TAG228TGG (20399T>C)
12810.4%12991.5%28 (100%)24 (85.7%)0/0/0/00
Protein mutations:
V13I (21123G>A) S22G (21150A>G 21152T>C) T26S (21162A>T 21164G>A)
Codon mutations:
GTA13ATA (21123G>A) ACC20ACT (21146C>T) AGT22GGC (21150A>G 21152T>C) ACG23ACT (21155G>T) TCT24TCC (21158T>C) GAA25GAG (21161A>G) ACG26TCA (21162A>T 21164G>A)
24141125.5%60587.9%105 (100%)99 (94.3%)0/0/2/01
Protein mutations:
S243A (22617T>G) T267N (22690C>A) A361S (22971G>T 22973A>T)
Codon mutations:
TCT243GCT (22617T>G) GAA245GAK (22625A>K) CAG246YWG (22626C>Y 22627A>W) ACC267AAC (22690C>A) TCC355TCT (22955C>T) GGC356GGT (22958C>T) GCT358GCA (22964T>A) AGC360AGT (22970C>T) GCA361TCT (22971G>T 22973A>T) GTC368GTA (22994C>A) GCT372GCG (23006T>G) GGC380GGT (23030C>T) GCG391GCT (23063G>T)
18543.8%59398.0%85 (100%)84 (98.8%)0/0/0/00
Protein mutations:
P9A (23147C>G)
Codon mutations:
CCA9GCA (23147C>G) CCG47CCA (23263G>A) CCG51CCT (23275G>T) CAT66CAC (23320T>C)
15546.6%34185.7%55 (100%)48 (87.3%)0/0/0/00
Protein mutations:
L2S (26344A>G) R8G (26327G>C) Q16E (26303G>C) S17P (26300A>G) W34R (26249A>G) I49V (26204T>C)
Codon mutations:
TTA2TCA (26344A>G) CGC8GGC (26327G>C) GTA13GTG (26310T>C) CAG16GAG (26303G>C) TCC17CCC (26300A>G) TAT28TAC (26265A>G) TGG34CGG (26249A>G) TTG40CTG (26231A>G) ACA46ACG (26211T>C) ATC49GTC (26204T>C)
325644.6%16896.6%25 (100%)25 (100%)0/0/0/01
Protein mutations:none
Codon mutations:
ACC42ACT (28385G>A)
173613.6%13190.3%20 (100%)19 (95.0%)0/0/0/00
Protein mutations:none
Codon mutations:none
477524.0%218100%29 (100%)29 (100%)0/0/0/00
Protein mutations:none
Codon mutations:
CTG74CTA (41492G>A)
358530.7%31687.5%51 (100%)45 (88.2%)0/0/0/00
Protein mutations:
E35G (44417A>G) T81I (44555C>T 44556G>A) V82G (44558T>G) A83P (44560G>C) R84K (44564G>A)
Codon mutations:
GAA35GGA (44417A>G) GTC37GTT (44424C>T) ACG81ATA (44555C>T 44556G>A) GTC82GGC (44558T>G) GCC83CCC (44560G>C) AGA84AAA (44564G>A)
178446.6%37582.6%68 (100%)59 (86.8%)0/0/0/00
Protein mutations:
N24D (44877A>G) H25N (44880C>A) N29D (44892A>G) M31I (44900G>C) T32V (44901A>G 44902C>T) E35V (44911A>T) D53N (44964G>A 44966C>T) D77A (45037A>C) R83H (45055G>A)
Codon mutations:
CTG19TTG (44862C>T) AAT24GAT (44877A>G) CAT25AAT (44880C>A) AAC29GAC (44892A>G) ATG31ATC (44900G>C) ACC32GTC (44901A>G 44902C>T) GAA35GTA (44911A>T) GAC38GAT (44921C>T) GAC46GAT (44945C>T) GAC53AAT (44964G>A 44966C>T) GCA55GCT (44972A>T) AAC69AAT (45014C>T) CTC75CTT (45032C>T) GAT77GCT (45037A>C) CGT83CAT (45055G>A)
17494.9%47196.1%73 (98.6%)70 (94.6%)0/1/0/00
Protein mutations:
T3del (47013_47015delCGT) I12T (46987A>G) D30V (46933T>A) I74V (46802T>C)
Codon mutations:
TCA2TCG (47016T>C) ACG3del (47013_47015delCGT) ATC12ACC (46987A>G) GAT30GTT (46933T>A) TCT56TCG (46854A>C) ATC74GTC (46802T>C)
156277.4%31891.4%48 (100%)42 (87.5%)0/0/0/01
Protein mutations:
N16D (47177T>C) V21I (47162C>T) I25V (47148G>C 47150T>C) Y26E (47145G>C 47147A>C) I41V (47102T>C) L48P (47080A>G)
Codon mutations:
AAT16GAT (47177T>C) CTG19CTA (47166C>T) GTC21ATC (47162C>T) ATC25GTG (47148G>C 47150T>C) TAC26GAG (47145G>C 47147A>C) ATA41GTA (47102T>C) CTT48CCT (47080A>G) TAA62TGA (47038T>C)
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43