Version 2.14.5
Genome Detective Platform

NGS Details (SRR3458562_Metagenome_example): Escherichia phage Lambda_ev099

Assembly

2403 (11 contig(s))
12.8
325
20.97 rpm (after QC)
3
de novo + reference guided assembly
Bcf Tools

Coverage Map

Variant Tables

Assignment

Escherichia phage Lambda_ev099 (Taxonomy ID: 2742953)
95.0417
91.3158
3
73

Alignment

4318.0 (NT) + 4807.0 (AA) = 9125.0
90.6967
Local, heuristic, nucleotide (BLASTN)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT20375471805.1%431890.2%2400 (99.9%)2281 (94.9%)0/3
Mutations:20399T>C, 20417T>A, 20422T>C, 20423C>T, 20429C>A, 20481T>G, 20495T>C, 20531C>T, 20576G>A, 20600A>G, 20611G>A, 20630G>A, 20632C>T, 20633G>A, 20708C>T, 20711C>T, 20717A>G, 20720C>T, 20728C>T, 20984T>A, 20997G>A, 20999A>G, 21008A>T, 21026A>G, 21035A>G, 21065T>G, 21123G>A, 21146C>T, 21150A>G, 21152T>C, 21155G>T, 21158T>C, 21161A>G, 21162A>T, 21164G>A, 22617T>G, 22625A>K, 22626C>Y, 22627A>W, 22690C>A, 22955C>T, 22958C>T, 22964T>A, 22970C>T, 22971G>T, 22973A>T, 22994C>A, 23006T>G, 23030C>T, 23063G>T, 23147C>G, 23263G>A, 23275G>T, 23320T>C, 26204T>C, 26211T>C, 26231A>G, 26249A>G, 26265A>G, 26300A>G, 26303G>C, 26310T>C, 26327G>C, 26344A>G, 26349G>C, 26358C>T, 26385G>A, 26388T>G, 26391G>T, 26400T>G, 26415G>A, 26417A>G, 26439A>C, 26442C>G, 26444G>C, 28330G>T, 28385G>A, 41492G>A, 44417A>G, 44424C>T, 44555C>T, 44556G>A, 44558T>G, 44560G>C, 44564G>A, 44862C>T, 44877A>G, 44880C>A, 44892A>G, 44900G>C, 44901A>G, 44902C>T, 44911A>T, 44921C>T, 44945C>T, 44964G>A, 44966C>T, 44972A>T, 45014C>T, 45032C>T, 45037A>C, 45055G>A, 46802T>C, 46854A>C, 46933T>A, 46987A>G, 47013_47015delCGT, 47016T>C, 47029T>C, 47032C>T, 47038T>C, 47080A>G, 47102T>C, 47145G>C, 47147A>C, 47148G>C, 47150T>C, 47162C>T, 47166C>T, 47177T>C
CDS
11623.5%8293.2%16 (100%)15 (93.8%)0/0/0/00
Protein mutations:M1V (20422T>C)
Codon mutations:ATG1GTG (20422T>C), GCA2GCT (20417T>A), GTA8GTG (20399T>C)
122868.9%106085.8%157 (100%)135 (86.0%)0/0/0/00
Protein mutations:H6P (21065T>G), L16P (21035A>G), L19P (21026A>G), V25E (21008A>T), L28P (20999A>G), P29S (20997G>A), Q33L (20984T>A), R121Q (20720C>T), M122T (20717A>G), R124Q (20711C>T), R125H (20708C>T), T150I (20632C>T 20633G>A), T151M (20630G>A), L161P (20600A>G), S169F (20576G>A), R184Q (20531C>T), Y196C (20495T>C), M201L (20481T>G), G218V (20429C>A), R220K (20422T>C 20423C>T), Q222L (20417T>A), *228W (20399T>C)
Codon mutations:CAC6CCC (21065T>G), CTG16CCG (21035A>G), CTG19CCG (21026A>G), GTG25GAG (21008A>T), CTG28CCG (20999A>G), CCG29TCG (20997G>A), CAG33CTG (20984T>A), CTG118CTA (20728C>T), CGG121CAG (20720C>T), ATG122ACG (20717A>G), CGG124CAG (20711C>T), CGC125CAC (20708C>T), ACG150ATA (20632C>T 20633G>A), ACG151ATG (20630G>A), AGC157AGT (20611G>A), CTG161CCG (20600A>G), TCT169TTT (20576G>A), CGG184CAG (20531C>T), TAC196TGC (20495T>C), ATG201CTG (20481T>G), GGG218GTG (20429C>A), AGA220AAG (20422T>C 20423C>T), CAG222CTG (20417T>A), TAG228TGG (20399T>C)
12810.4%12991.5%28 (100%)24 (85.7%)0/0/0/00
Protein mutations:V13I (21123G>A), S22G (21150A>G 21152T>C), T26S (21162A>T 21164G>A)
Codon mutations:GTA13ATA (21123G>A), ACC20ACT (21146C>T), AGT22GGC (21150A>G 21152T>C), ACG23ACT (21155G>T), TCT24TCC (21158T>C), GAA25GAG (21161A>G), ACG26TCA (21162A>T 21164G>A)
24141125.5%60587.9%105 (100%)99 (94.3%)0/0/2/01
Protein mutations:S243A (22617T>G), T267N (22690C>A), A361S (22971G>T 22973A>T)
Codon mutations:TCT243GCT (22617T>G), GAA245GAK (22625A>K), CAG246YWG (22626C>Y 22627A>W), ACC267AAC (22690C>A), TCC355TCT (22955C>T), GGC356GGT (22958C>T), GCT358GCA (22964T>A), AGC360AGT (22970C>T), GCA361TCT (22971G>T 22973A>T), GTC368GTA (22994C>A), GCT372GCG (23006T>G), GGC380GGT (23030C>T), GCG391GCT (23063G>T)
18543.8%59398.0%85 (100%)84 (98.8%)0/0/0/00
Protein mutations:P9A (23147C>G)
Codon mutations:CCA9GCA (23147C>G), CCG47CCA (23263G>A), CCG51CCT (23275G>T), CAT66CAC (23320T>C)
15546.6%34185.7%55 (100%)48 (87.3%)0/0/0/00
Protein mutations:L2S (26344A>G), R8G (26327G>C), Q16E (26303G>C), S17P (26300A>G), W34R (26249A>G), I49V (26204T>C)
Codon mutations:TTA2TCA (26344A>G), CGC8GGC (26327G>C), GTA13GTG (26310T>C), CAG16GAG (26303G>C), TCC17CCC (26300A>G), TAT28TAC (26265A>G), TGG34CGG (26249A>G), TTG40CTG (26231A>G), ACA46ACG (26211T>C), ATC49GTC (26204T>C)
325644.6%16896.6%25 (100%)25 (100%)0/0/0/01
173613.6%13190.3%20 (100%)19 (95.0%)0/0/0/00
477524.0%218100%29 (100%)29 (100%)0/0/0/00
358530.7%31687.5%51 (100%)45 (88.2%)0/0/0/00
Protein mutations:E35G (44417A>G), T81I (44555C>T 44556G>A), V82G (44558T>G), A83P (44560G>C), R84K (44564G>A)
Codon mutations:GAA35GGA (44417A>G), GTC37GTT (44424C>T), ACG81ATA (44555C>T 44556G>A), GTC82GGC (44558T>G), GCC83CCC (44560G>C), AGA84AAA (44564G>A)
178446.6%37582.6%68 (100%)59 (86.8%)0/0/0/00
Protein mutations:N24D (44877A>G), H25N (44880C>A), N29D (44892A>G), M31I (44900G>C), T32V (44901A>G 44902C>T), E35V (44911A>T), D53N (44964G>A 44966C>T), D77A (45037A>C), R83H (45055G>A)
Codon mutations:CTG19TTG (44862C>T), AAT24GAT (44877A>G), CAT25AAT (44880C>A), AAC29GAC (44892A>G), ATG31ATC (44900G>C), ACC32GTC (44901A>G 44902C>T), GAA35GTA (44911A>T), GAC38GAT (44921C>T), GAC46GAT (44945C>T), GAC53AAT (44964G>A 44966C>T), GCA55GCT (44972A>T), AAC69AAT (45014C>T), CTC75CTT (45032C>T), GAT77GCT (45037A>C), CGT83CAT (45055G>A)
17494.9%47196.1%73 (98.6%)70 (94.6%)0/1/0/00
Protein mutations:T3del (47013_47015delCGT), I12T (46987A>G), D30V (46933T>A), I74V (46802T>C)
Codon mutations:TCA2TCG (47016T>C), ACG3del (47013_47015delCGT), ATC12ACC (46987A>G), GAT30GTT (46933T>A), TCT56TCG (46854A>C), ATC74GTC (46802T>C)
156277.4%31891.4%48 (100%)42 (87.5%)0/0/0/01
Protein mutations:N16D (47177T>C), V21I (47162C>T), I25V (47148G>C 47150T>C), Y26E (47145G>C 47147A>C), I41V (47102T>C), L48P (47080A>G)
Codon mutations:AAT16GAT (47177T>C), CTG19CTA (47166C>T), GTC21ATC (47162C>T), ATC25GTG (47148G>C 47150T>C), TAC26GAG (47145G>C 47147A>C), ATA41GTA (47102T>C), CTT48CCT (47080A>G), TAA62TGA (47038T>C)
Proteins
11623.5%8293.2%16 (100%)15 (93.8%)0/0/0/00
Protein mutations:M1V (20422T>C)
Codon mutations:ATG1GTG (20422T>C), GCA2GCT (20417T>A), GTA8GTG (20399T>C)
122868.9%106085.8%157 (100%)135 (86.0%)0/0/0/00
Protein mutations:H6P (21065T>G), L16P (21035A>G), L19P (21026A>G), V25E (21008A>T), L28P (20999A>G), P29S (20997G>A), Q33L (20984T>A), R121Q (20720C>T), M122T (20717A>G), R124Q (20711C>T), R125H (20708C>T), T150I (20632C>T 20633G>A), T151M (20630G>A), L161P (20600A>G), S169F (20576G>A), R184Q (20531C>T), Y196C (20495T>C), M201L (20481T>G), G218V (20429C>A), R220K (20422T>C 20423C>T), Q222L (20417T>A), *228W (20399T>C)
Codon mutations:CAC6CCC (21065T>G), CTG16CCG (21035A>G), CTG19CCG (21026A>G), GTG25GAG (21008A>T), CTG28CCG (20999A>G), CCG29TCG (20997G>A), CAG33CTG (20984T>A), CTG118CTA (20728C>T), CGG121CAG (20720C>T), ATG122ACG (20717A>G), CGG124CAG (20711C>T), CGC125CAC (20708C>T), ACG150ATA (20632C>T 20633G>A), ACG151ATG (20630G>A), AGC157AGT (20611G>A), CTG161CCG (20600A>G), TCT169TTT (20576G>A), CGG184CAG (20531C>T), TAC196TGC (20495T>C), ATG201CTG (20481T>G), GGG218GTG (20429C>A), AGA220AAG (20422T>C 20423C>T), CAG222CTG (20417T>A), TAG228TGG (20399T>C)
12810.4%12991.5%28 (100%)24 (85.7%)0/0/0/00
Protein mutations:V13I (21123G>A), S22G (21150A>G 21152T>C), T26S (21162A>T 21164G>A)
Codon mutations:GTA13ATA (21123G>A), ACC20ACT (21146C>T), AGT22GGC (21150A>G 21152T>C), ACG23ACT (21155G>T), TCT24TCC (21158T>C), GAA25GAG (21161A>G), ACG26TCA (21162A>T 21164G>A)
24141125.5%60587.9%105 (100%)99 (94.3%)0/0/2/01
Protein mutations:S243A (22617T>G), T267N (22690C>A), A361S (22971G>T 22973A>T)
Codon mutations:TCT243GCT (22617T>G), GAA245GAK (22625A>K), CAG246YWG (22626C>Y 22627A>W), ACC267AAC (22690C>A), TCC355TCT (22955C>T), GGC356GGT (22958C>T), GCT358GCA (22964T>A), AGC360AGT (22970C>T), GCA361TCT (22971G>T 22973A>T), GTC368GTA (22994C>A), GCT372GCG (23006T>G), GGC380GGT (23030C>T), GCG391GCT (23063G>T)
18543.8%59398.0%85 (100%)84 (98.8%)0/0/0/00
Protein mutations:P9A (23147C>G)
Codon mutations:CCA9GCA (23147C>G), CCG47CCA (23263G>A), CCG51CCT (23275G>T), CAT66CAC (23320T>C)
15546.6%34185.7%55 (100%)48 (87.3%)0/0/0/00
Protein mutations:L2S (26344A>G), R8G (26327G>C), Q16E (26303G>C), S17P (26300A>G), W34R (26249A>G), I49V (26204T>C)
Codon mutations:TTA2TCA (26344A>G), CGC8GGC (26327G>C), GTA13GTG (26310T>C), CAG16GAG (26303G>C), TCC17CCC (26300A>G), TAT28TAC (26265A>G), TGG34CGG (26249A>G), TTG40CTG (26231A>G), ACA46ACG (26211T>C), ATC49GTC (26204T>C)
325644.6%16896.6%25 (100%)25 (100%)0/0/0/01
Protein mutations:none
Codon mutations:ACC42ACT (28385G>A)
173613.6%13190.3%20 (100%)19 (95.0%)0/0/0/00
Protein mutations:none
Codon mutations:none
477524.0%218100%29 (100%)29 (100%)0/0/0/00
Protein mutations:none
Codon mutations:CTG74CTA (41492G>A)
358530.7%31687.5%51 (100%)45 (88.2%)0/0/0/00
Protein mutations:E35G (44417A>G), T81I (44555C>T 44556G>A), V82G (44558T>G), A83P (44560G>C), R84K (44564G>A)
Codon mutations:GAA35GGA (44417A>G), GTC37GTT (44424C>T), ACG81ATA (44555C>T 44556G>A), GTC82GGC (44558T>G), GCC83CCC (44560G>C), AGA84AAA (44564G>A)
178446.6%37582.6%68 (100%)59 (86.8%)0/0/0/00
Protein mutations:N24D (44877A>G), H25N (44880C>A), N29D (44892A>G), M31I (44900G>C), T32V (44901A>G 44902C>T), E35V (44911A>T), D53N (44964G>A 44966C>T), D77A (45037A>C), R83H (45055G>A)
Codon mutations:CTG19TTG (44862C>T), AAT24GAT (44877A>G), CAT25AAT (44880C>A), AAC29GAC (44892A>G), ATG31ATC (44900G>C), ACC32GTC (44901A>G 44902C>T), GAA35GTA (44911A>T), GAC38GAT (44921C>T), GAC46GAT (44945C>T), GAC53AAT (44964G>A 44966C>T), GCA55GCT (44972A>T), AAC69AAT (45014C>T), CTC75CTT (45032C>T), GAT77GCT (45037A>C), CGT83CAT (45055G>A)
17494.9%47196.1%73 (98.6%)70 (94.6%)0/1/0/00
Protein mutations:T3del (47013_47015delCGT), I12T (46987A>G), D30V (46933T>A), I74V (46802T>C)
Codon mutations:TCA2TCG (47016T>C), ACG3del (47013_47015delCGT), ATC12ACC (46987A>G), GAT30GTT (46933T>A), TCT56TCG (46854A>C), ATC74GTC (46802T>C)
156277.4%31891.4%48 (100%)42 (87.5%)0/0/0/01
Protein mutations:N16D (47177T>C), V21I (47162C>T), I25V (47148G>C 47150T>C), Y26E (47145G>C 47147A>C), I41V (47102T>C), L48P (47080A>G)
Codon mutations:AAT16GAT (47177T>C), CTG19CTA (47166C>T), GTC21ATC (47162C>T), ATC25GTG (47148G>C 47150T>C), TAC26GAG (47145G>C 47147A>C), ATA41GTA (47102T>C), CTT48CCT (47080A>G), TAA62TGA (47038T>C)
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43