NGS Details (SRR3458562_Metagenome_example): Betapolyomavirus hominis

Assembly

5148 (1 contig(s))
3512.0
169687
10948.96 rpm (after QC)
0
read mapping against reference + variant calling
Bcf Tools

Coverage Map

Variant Tables

Assignment

Betapolyomavirus hominis (Taxonomy ID: 1891762)
98.8072
99.6815
6
6

Alignment

10189.0 (NT) + 13224.0 (AA) = 23413.0
99.4351
Global, seeded, nucleotide + amino acids (AGA)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT1515399.9%1018999.0%5148 (99.0%)5136 (98.8%)50/0
Mutations:327_328insTGGGCAGCCAGCCAGTGGCAGTTAATAGTGAAACCGGAAAGCAGCCAGAC, 905A>G, 922A>G, 930G>A, 931A>G, 2028A>T, 2037A>C, 2074T>A, 2085T>A, 2193A>T, 2218G>A, 3472A>T, 4031C>T
CDS
167100%416100%67 (100%)67 (100%)0/0/0/01
1352100%232399.5%352 (100%)350 (99.4%)0/0/0/01
Protein mutations:K100R (922A>G), D103S (930G>A 931A>G)
Codon mutations:TTA94TTG (905A>G), AAG100AGG (922A>G), GAT103AGT (930G>A 931A>G)
1233100%1632100%233 (100%)233 (100%)0/0/0/01
1363100%254499.6%363 (100%)360 (99.2%)0/0/0/01
Protein mutations:E158D (2037A>C), S171T (2074T>A), A219T (2218G>A)
Codon mutations:GTA155GTT (2028A>T), GAA158GAC (2037A>C), TCA171ACA (2074T>A), CCT174CCA (2085T>A), GTA210GTT (2193A>T), GCT219ACT (2218G>A)
1696100%495499.9%696 (100%)695 (99.9%)0/0/0/01
Protein mutations:S260N (4031C>T)
Codon mutations:AGC260AAC (4031C>T), GGT446GGA (3472A>T)
1173100%1355100%173 (100%)173 (100%)0/0/0/01
Proteins
167100%416100%67 (100%)67 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
1352100%232399.5%352 (100%)350 (99.4%)0/0/0/01
Protein mutations:K100R (922A>G), D103S (930G>A 931A>G)
Codon mutations:TTA94TTG (905A>G), AAG100AGG (922A>G), GAT103AGT (930G>A 931A>G)
1233100%1632100%233 (100%)233 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
1363100%254499.6%363 (100%)360 (99.2%)0/0/0/01
Protein mutations:E158D (2037A>C), S171T (2074T>A), A219T (2218G>A)
Codon mutations:GTA155GTT (2028A>T), GAA158GAC (2037A>C), TCA171ACA (2074T>A), CCT174CCA (2085T>A), GTA210GTT (2193A>T), GCT219ACT (2218G>A)
1696100%495499.9%696 (100%)695 (99.9%)0/0/0/01
Protein mutations:S260N (4031C>T)
Codon mutations:AGC260AAC (4031C>T), GGT446GGA (3472A>T)
1173100%1355100%173 (100%)173 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43