NGS Details (SRR3458562_Metagenome_example): Escherichia phage Lambda_ev017

Assembly

40978 (59 contig(s))
10.1
3798
245.06 rpm (after QC)
0
read mapping against reference + variant calling
Bcf Tools

Coverage Map

Variant Tables

Assignment

Escherichia phage Lambda_ev017 (Taxonomy ID: 2742949)
99.9488
99.5587
63
73

Alignment

81872.0 (NT) + 81357.0 (AA) = 163229.0
99.899
Local, heuristic, nucleotide (BLASTN)

Alignment Detailed Statistics

BeginEndCoverageScoreConcordanceMatchesIdentitiesI/D/M/F*Stop Codons
NT165006981.8%8187299.9%40978 (100%)40957 (99.9%)0/0
Mutations:469A>C, 3544G>T, 3613G>A, 5114T>A, 6118G>T, 7221T>C, 8701T>C, 9265A>G, 10299T>C, 10534G>A, 11419A>G, 13350T>C, 14108C>T, 16002A>T, 18864A>T, 19808G>C, 21089A>G, 23045C>T, 23124A>G, 47809C>T, 48176T>G
CDS
1183100%119199.3%183 (100%)182 (99.5%)0/0/0/01
Protein mutations:D67A (469A>C)
Codon mutations:GAC67GCC (469A>C)
164396.1%440899.6%618 (100%)617 (99.8%)0/0/0/01
56994.2%37892.6%65 (100%)64 (98.5%)0/0/1/01
153188.7%325199.1%471 (100%)469 (99.6%)0/0/0/02
Protein mutations:G214* (3544G>T), V237M (3613G>A)
Codon mutations:GGA214TGA (3544G>T), GTG237ATG (3613G>A)
150282.3%264399.1%413 (100%)410 (99.3%)0/0/0/01
Protein mutations:S210T (5114T>A)
Codon mutations:TCA210ACA (5114T>A)
1116100%744100%116 (100%)116 (100%)0/0/0/01
1343100%232099.7%343 (100%)342 (99.7%)0/0/0/01
Protein mutations:I263T (7221T>C)
Codon mutations:ATC263ACC (7221T>C)
1125100%805100%125 (100%)125 (100%)0/0/0/01
111885.6%73198.3%101 (100%)100 (99.0%)0/0/0/01
119275.1%92497.9%145 (100%)143 (98.6%)0/0/0/00
9013232.6%28095.9%43 (100%)43 (100%)0/0/0/01
124090.7%146698.9%224 (100%)224 (100%)0/0/0/00
2114185.8%80098.6%121 (100%)120 (99.2%)0/0/0/01
Protein mutations:L107P (10299T>C)
Codon mutations:CTG107CCG (10299T>C)
114586.9%89798.6%126 (100%)125 (99.2%)0/0/0/01
Protein mutations:D51N (10534G>A)
Codon mutations:GAT51AAT (10534G>A)
185470.0%380498.7%598 (100%)594 (99.3%)0/0/0/01
Protein mutations:L847P (13350T>C)
Codon mutations:GCA203GCG (11419A>G), CTG847CCG (13350T>C)
1110100%752100%110 (100%)110 (100%)0/0/0/01
1233100%1667100%233 (100%)233 (100%)0/0/0/01
124888.7%173499.3%220 (100%)219 (99.5%)0/0/0/01
1191100%1223100%191 (100%)191 (100%)0/0/0/01
1116186.6%688899.0%1006 (100%)1002 (99.6%)0/0/0/01
168796.5%430399.4%663 (100%)659 (99.4%)0/0/0/01
Protein mutations:A165P (19808G>C), S592G (21089A>G)
Codon mutations:GCA165CCA (19808G>C), AGT592GGT (21089A>G)
193100%643100%93 (100%)93 (100%)0/0/0/01
198100%761100%98 (100%)98 (100%)0/0/0/01
1285100%1861100%285 (100%)285 (100%)0/0/0/01
128695.1%179698.8%272 (100%)271 (99.6%)0/0/0/01
Protein mutations:S239N (23045C>T)
Codon mutations:TTA213CTA (23124A>G), AGT239AAT (23045C>T)
127697.5%168496.9%269 (100%)266 (98.9%)0/0/2/01
130593.4%188699.1%285 (100%)284 (99.6%)0/0/0/01
142100%286100%42 (100%)42 (100%)0/0/0/01
125395.7%163199.4%242 (100%)241 (99.6%)0/0/0/01
43970.6%20083.7%36 (100%)35 (97.2%)0/0/0/00
3330548.5%102294.0%148 (100%)146 (98.6%)0/0/1/01
237972.2%39798.5%57 (100%)57 (100%)0/0/0/01
39554.7%34990.4%52 (100%)50 (96.2%)0/0/0/01
2472470.4%111.1%1 (100%)1 (100%)0/0/0/01
12331.9%13397.1%23 (100%)22 (95.7%)0/0/0/00
406431.3%8988.1%20 (100%)19 (95.0%)0/0/0/01
12310.1%15397.5%23 (100%)22 (95.7%)0/0/0/00
126252.3%92197.4%137 (100%)136 (99.3%)0/0/0/01
199100%705100%99 (100%)99 (100%)0/0/0/01
143100%268100%43 (100%)43 (100%)0/0/0/01
155100%408100%55 (100%)55 (100%)0/0/0/01
1123100%877100%123 (100%)123 (100%)0/0/0/01
141100%265100%41 (100%)41 (100%)0/0/0/01
118587.0%104997.3%161 (100%)159 (98.8%)0/0/0/01
141100%244100%41 (100%)41 (100%)0/0/0/01
1251100%1682100%251 (100%)251 (100%)0/0/0/01
118695.7%134598.5%178 (100%)177 (99.4%)0/0/0/01
1232100%1629100%232 (100%)232 (100%)0/0/0/01
172100%493100%72 (100%)72 (100%)0/0/0/01
199100%643100%99 (100%)99 (100%)0/0/0/01
130098.0%206999.6%294 (100%)293 (99.7%)0/0/0/01
1234100%1608100%234 (100%)234 (100%)0/0/0/01
197100%621100%97 (100%)97 (100%)0/0/0/01
1147100%1053100%147 (100%)147 (100%)0/0/0/01
1176100%1287100%176 (100%)176 (100%)0/0/0/01
111495.6%78599.2%109 (100%)108 (99.1%)0/0/0/01
1121100%854100%121 (100%)121 (100%)0/0/0/01
12961.7%20497.6%29 (100%)28 (96.6%)0/0/0/00
112893.0%85699.0%119 (100%)118 (99.2%)0/0/0/01
2838188.5%229599.3%337 (100%)336 (99.7%)0/0/0/01
172100%451100%72 (100%)72 (100%)0/0/0/01
1166100%1158100%166 (100%)166 (100%)0/0/0/01
1154100%99699.7%154 (100%)153 (99.4%)0/0/0/01
Protein mutations:A50V (47809C>T)
Codon mutations:GCA50GTA (47809C>T)
110394.2%63497.7%97 (100%)95 (97.9%)0/0/0/01
Protein mutations:E96A (48176T>G)
Codon mutations:GAG96GCG (48176T>G)
146100%314100%46 (100%)46 (100%)0/0/0/01
180100%570100%80 (100%)80 (100%)0/0/0/01
161100%403100%61 (100%)61 (100%)0/0/0/01
177100%569100%77 (100%)77 (100%)0/0/0/01
Proteins
1183100%119199.3%183 (100%)182 (99.5%)0/0/0/01
Protein mutations:D67A (469A>C)
Codon mutations:GAC67GCC (469A>C)
164396.1%440899.6%618 (100%)617 (99.8%)0/0/0/01
Protein mutations:none
Codon mutations:none
56994.2%37892.6%65 (100%)64 (98.5%)0/0/1/01
Protein mutations:none
Codon mutations:none
153188.7%325199.1%471 (100%)469 (99.6%)0/0/0/02
Protein mutations:G214* (3544G>T), V237M (3613G>A)
Codon mutations:GGA214TGA (3544G>T), GTG237ATG (3613G>A)
150282.3%264399.1%413 (100%)410 (99.3%)0/0/0/01
Protein mutations:S210T (5114T>A)
Codon mutations:TCA210ACA (5114T>A)
1116100%744100%116 (100%)116 (100%)0/0/0/01
Protein mutations:none
Codon mutations:GGG30GGT (6118G>T)
1343100%232099.7%343 (100%)342 (99.7%)0/0/0/01
Protein mutations:I263T (7221T>C)
Codon mutations:ATC263ACC (7221T>C)
1125100%805100%125 (100%)125 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
111885.6%73198.3%101 (100%)100 (99.0%)0/0/0/01
Protein mutations:none
Codon mutations:none
119275.1%92497.9%145 (100%)143 (98.6%)0/0/0/00
Protein mutations:none
Codon mutations:ATT152ATC (8701T>C)
9013232.6%28095.9%43 (100%)43 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
124090.7%146698.9%224 (100%)224 (100%)0/0/0/00
Protein mutations:none
Codon mutations:GGA14GGG (9265A>G)
2114185.8%80098.6%121 (100%)120 (99.2%)0/0/0/01
Protein mutations:L107P (10299T>C)
Codon mutations:CTG107CCG (10299T>C)
114586.9%89798.6%126 (100%)125 (99.2%)0/0/0/01
Protein mutations:D51N (10534G>A)
Codon mutations:GAT51AAT (10534G>A)
185470.0%380498.7%598 (100%)594 (99.3%)0/0/0/01
Protein mutations:L847P (13350T>C)
Codon mutations:GCA203GCG (11419A>G), CTG847CCG (13350T>C)
1110100%752100%110 (100%)110 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
1233100%1667100%233 (100%)233 (100%)0/0/0/01
Protein mutations:none
Codon mutations:ATC137ATT (14108C>T)
124888.7%173499.3%220 (100%)219 (99.5%)0/0/0/01
Protein mutations:none
Codon mutations:none
1191100%1223100%191 (100%)191 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
1116186.6%688899.0%1006 (100%)1002 (99.6%)0/0/0/01
Protein mutations:none
Codon mutations:ACA76ACT (16002A>T), GGA1030GGT (18864A>T)
168796.5%430399.4%663 (100%)659 (99.4%)0/0/0/01
Protein mutations:A165P (19808G>C), S592G (21089A>G)
Codon mutations:GCA165CCA (19808G>C), AGT592GGT (21089A>G)
193100%643100%93 (100%)93 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
198100%761100%98 (100%)98 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
1285100%1861100%285 (100%)285 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
128695.1%179698.8%272 (100%)271 (99.6%)0/0/0/01
Protein mutations:S239N (23045C>T)
Codon mutations:TTA213CTA (23124A>G), AGT239AAT (23045C>T)
127697.5%168496.9%269 (100%)266 (98.9%)0/0/2/01
Protein mutations:none
Codon mutations:none
130593.4%188699.1%285 (100%)284 (99.6%)0/0/0/01
Protein mutations:none
Codon mutations:none
142100%286100%42 (100%)42 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
125395.7%163199.4%242 (100%)241 (99.6%)0/0/0/01
Protein mutations:none
Codon mutations:none
43970.6%20083.7%36 (100%)35 (97.2%)0/0/0/00
Protein mutations:none
Codon mutations:none
3330548.5%102294.0%148 (100%)146 (98.6%)0/0/1/01
Protein mutations:none
Codon mutations:none
237972.2%39798.5%57 (100%)57 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
39554.7%34990.4%52 (100%)50 (96.2%)0/0/0/01
Protein mutations:none
Codon mutations:none
2472470.4%111.1%1 (100%)1 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
12331.9%13397.1%23 (100%)22 (95.7%)0/0/0/00
Protein mutations:none
Codon mutations:none
406431.3%8988.1%20 (100%)19 (95.0%)0/0/0/01
Protein mutations:none
Codon mutations:none
12310.1%15397.5%23 (100%)22 (95.7%)0/0/0/00
Protein mutations:none
Codon mutations:none
126252.3%92197.4%137 (100%)136 (99.3%)0/0/0/01
Protein mutations:none
Codon mutations:none
199100%705100%99 (100%)99 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
143100%268100%43 (100%)43 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
155100%408100%55 (100%)55 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
1123100%877100%123 (100%)123 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
141100%265100%41 (100%)41 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
118587.0%104997.3%161 (100%)159 (98.8%)0/0/0/01
Protein mutations:none
Codon mutations:none
141100%244100%41 (100%)41 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
1251100%1682100%251 (100%)251 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
118695.7%134598.5%178 (100%)177 (99.4%)0/0/0/01
Protein mutations:none
Codon mutations:none
1232100%1629100%232 (100%)232 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
172100%493100%72 (100%)72 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
199100%643100%99 (100%)99 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
130098.0%206999.6%294 (100%)293 (99.7%)0/0/0/01
Protein mutations:none
Codon mutations:none
1234100%1608100%234 (100%)234 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
197100%621100%97 (100%)97 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
1147100%1053100%147 (100%)147 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
1176100%1287100%176 (100%)176 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
111495.6%78599.2%109 (100%)108 (99.1%)0/0/0/01
Protein mutations:none
Codon mutations:none
1121100%854100%121 (100%)121 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
12961.7%20497.6%29 (100%)28 (96.6%)0/0/0/00
Protein mutations:none
Codon mutations:none
112893.0%85699.0%119 (100%)118 (99.2%)0/0/0/01
Protein mutations:none
Codon mutations:none
2838188.5%229599.3%337 (100%)336 (99.7%)0/0/0/01
Protein mutations:none
Codon mutations:none
172100%451100%72 (100%)72 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
1166100%1158100%166 (100%)166 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
1154100%99699.7%154 (100%)153 (99.4%)0/0/0/01
Protein mutations:A50V (47809C>T)
Codon mutations:GCA50GTA (47809C>T)
110394.2%63497.7%97 (100%)95 (97.9%)0/0/0/01
Protein mutations:E96A (48176T>G)
Codon mutations:GAG96GCG (48176T>G)
146100%314100%46 (100%)46 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
180100%570100%80 (100%)80 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
161100%403100%61 (100%)61 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
177100%569100%77 (100%)77 (100%)0/0/0/01
Protein mutations:none
Codon mutations:none
*: Inserts / Deletes / Misaligned / Frameshifts

Analysis details

This analysis was performed with panviral2.43